Validating Transcripts with Probes and Imaging Technology
High-throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples. However, the analysis of spatial gene expression patterns with single-cell resolution requires quantitative in situ measurement techniques. Here we describe recent techno...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2012
|
| Online Access: | http://hdl.handle.net/1721.1/71013 |
| _version_ | 1826195483374125056 |
|---|---|
| author | Itzkovitz, Shaul Shalev van Oudenaarden, Alexander |
| author2 | MIT Materials Research Laboratory |
| author_facet | MIT Materials Research Laboratory Itzkovitz, Shaul Shalev van Oudenaarden, Alexander |
| author_sort | Itzkovitz, Shaul Shalev |
| collection | MIT |
| description | High-throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples. However, the analysis of spatial gene expression patterns with single-cell resolution requires quantitative in situ measurement techniques. Here we describe recent technological advances in RNA fluorescence in situ hybridization (FISH) techniques that facilitate detection of individual fluorescently labeled mRNA molecules of practically any endogenous gene. These methods, which are based on advances in probe design, imaging technology and image processing, enable the absolute measurement of transcript abundance in individual cells with single-molecule resolution. |
| first_indexed | 2024-09-23T10:13:23Z |
| format | Article |
| id | mit-1721.1/71013 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:13:23Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/710132022-09-26T16:32:42Z Validating Transcripts with Probes and Imaging Technology Itzkovitz, Shaul Shalev van Oudenaarden, Alexander MIT Materials Research Laboratory Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics Koch Institute for Integrative Cancer Research at MIT van Oudenaarden, Alexander Itzkovitz, Shaul Shalev van Oudenaarden, Alexander High-throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples. However, the analysis of spatial gene expression patterns with single-cell resolution requires quantitative in situ measurement techniques. Here we describe recent technological advances in RNA fluorescence in situ hybridization (FISH) techniques that facilitate detection of individual fluorescently labeled mRNA molecules of practically any endogenous gene. These methods, which are based on advances in probe design, imaging technology and image processing, enable the absolute measurement of transcript abundance in individual cells with single-molecule resolution. National Cancer Institute (U.S.). Physical Sciences-Oncology Center (U54CA143874) National Institutes of Health (U.S.) (Pioneer award 1DP1OD003936) 2012-06-04T17:24:57Z 2012-06-04T17:24:57Z 2011-03 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/71013 Itzkovitz, Shalev, and Alexander van Oudenaarden. “Validating transcripts with probes and imaging technology.” Nature Methods 8.4s (2011): S12-S19. en_US http://dx.doi.org/10.1038/nmeth.1573 Nature Methods Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group Prof. van Oudenaarden via Mat Wilmott |
| spellingShingle | Itzkovitz, Shaul Shalev van Oudenaarden, Alexander Validating Transcripts with Probes and Imaging Technology |
| title | Validating Transcripts with Probes and Imaging Technology |
| title_full | Validating Transcripts with Probes and Imaging Technology |
| title_fullStr | Validating Transcripts with Probes and Imaging Technology |
| title_full_unstemmed | Validating Transcripts with Probes and Imaging Technology |
| title_short | Validating Transcripts with Probes and Imaging Technology |
| title_sort | validating transcripts with probes and imaging technology |
| url | http://hdl.handle.net/1721.1/71013 |
| work_keys_str_mv | AT itzkovitzshaulshalev validatingtranscriptswithprobesandimagingtechnology AT vanoudenaardenalexander validatingtranscriptswithprobesandimagingtechnology |