Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image f...

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Bibliographic Details
Main Authors: Dasari, Ramachandra Rao, Feld, Michael S., Sung, Yongjin, Kim, Moonseok, Choi, Youngwoon, Fang-Yen, Chris, Kim, Kwanhyung, Choi, Wonshik
Other Authors: Massachusetts Institute of Technology. Spectroscopy Laboratory
Format: Article
Language:en_US
Published: SPIE 2012
Online Access:http://hdl.handle.net/1721.1/71243
Description
Summary:We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

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