Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function
Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function, and dysfunction. We used a Bacterial Artificial Chromosome (BAC) transgenic strategy to express Channelrhodopsin2 (ChR2) under the control of cell-type specific promoter elements. We provide a de...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/71248 https://orcid.org/0000-0002-8021-277X https://orcid.org/0000-0002-4326-7720 |
| _version_ | 1811095339285872640 |
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| author | Zhao, Shengli Ting, Jonathan Thomas Atallah, Hicham Qiu, Li Tan, Jie Gloss, Bernd Augustine, George J. Deisseroth, Karl Luo, Minmin Graybiel, Ann M. Feng, Guoping |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Zhao, Shengli Ting, Jonathan Thomas Atallah, Hicham Qiu, Li Tan, Jie Gloss, Bernd Augustine, George J. Deisseroth, Karl Luo, Minmin Graybiel, Ann M. Feng, Guoping |
| author_sort | Zhao, Shengli |
| collection | MIT |
| description | Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function, and dysfunction. We used a Bacterial Artificial Chromosome (BAC) transgenic strategy to express Channelrhodopsin2 (ChR2) under the control of cell-type specific promoter elements. We provide a detailed functional characterization of the newly established VGAT-ChR2-EYFP, ChAT-ChR2-EYFP, TPH2-ChR2-EYFP and Pvalb-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action potential firing of GABAergic, cholinergic, serotonergic, and parvalbumin+ neuron subsets using blue light. This resource of cell type-specific ChR2 mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior. |
| first_indexed | 2024-09-23T16:15:31Z |
| format | Article |
| id | mit-1721.1/71248 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:15:31Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/712482022-04-01T17:13:09Z Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function Zhao, Shengli Ting, Jonathan Thomas Atallah, Hicham Qiu, Li Tan, Jie Gloss, Bernd Augustine, George J. Deisseroth, Karl Luo, Minmin Graybiel, Ann M. Feng, Guoping Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Feng, Guoping Feng, Guoping Atallah, Hicham Ting, Jonathan Thomas Graybiel, Ann M. Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function, and dysfunction. We used a Bacterial Artificial Chromosome (BAC) transgenic strategy to express Channelrhodopsin2 (ChR2) under the control of cell-type specific promoter elements. We provide a detailed functional characterization of the newly established VGAT-ChR2-EYFP, ChAT-ChR2-EYFP, TPH2-ChR2-EYFP and Pvalb-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action potential firing of GABAergic, cholinergic, serotonergic, and parvalbumin+ neuron subsets using blue light. This resource of cell type-specific ChR2 mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior. National Institutes of Health (U.S.) (Ruth L. Kirschstein National Research Service award (F32MH084460)) National Institute of Mental Health (U.S.) (RC1-MH088434) Brain and Behavior Research Foundation (Young Investigator award) 2012-06-28T13:38:15Z 2012-06-28T13:38:15Z 2011-10 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/71248 Zhao, Shengli et al. "Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function." Nature Methods, 2011 ; 8(9): 745–752. https://orcid.org/0000-0002-8021-277X https://orcid.org/0000-0002-4326-7720 en_US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191888/pdf/nihms-311754.pdf?tool=pmcentrez Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PubMed Central |
| spellingShingle | Zhao, Shengli Ting, Jonathan Thomas Atallah, Hicham Qiu, Li Tan, Jie Gloss, Bernd Augustine, George J. Deisseroth, Karl Luo, Minmin Graybiel, Ann M. Feng, Guoping Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function |
| title | Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function |
| title_full | Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function |
| title_fullStr | Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function |
| title_full_unstemmed | Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function |
| title_short | Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function |
| title_sort | cell type specific optogenetic mice for dissecting neural circuitry function |
| url | http://hdl.handle.net/1721.1/71248 https://orcid.org/0000-0002-8021-277X https://orcid.org/0000-0002-4326-7720 |
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