A novel method for mammalian large genetic circuit assembly and delivery
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, February 2012.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2012
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| Online Access: | http://hdl.handle.net/1721.1/71499 |
| _version_ | 1811090015833292800 |
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| author | Li, Yinqing, Ph. D. Massachusetts Institute of Technology |
| author2 | Ron Weiss. |
| author_facet | Ron Weiss. Li, Yinqing, Ph. D. Massachusetts Institute of Technology |
| author_sort | Li, Yinqing, Ph. D. Massachusetts Institute of Technology |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, February 2012. |
| first_indexed | 2024-09-23T14:30:17Z |
| format | Thesis |
| id | mit-1721.1/71499 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:30:17Z |
| publishDate | 2012 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/714992019-04-11T00:28:57Z A novel method for mammalian large genetic circuit assembly and delivery Li, Yinqing, Ph. D. Massachusetts Institute of Technology Ron Weiss. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, February 2012. "February 2012." Cataloged from PDF version of thesis. Includes bibliographical references (p. 65-75). Genetic manipulation of mammalian cells provides a foundation for contemporary biological research both basic and applied. Existing methods for construction and introduction of large scale exogenous genetic information into mammalian cells and for creating stable cell lines are not efficient and suffer from limitations in terms of cost, speed, flexibility, and reliability. In this thesis, a novel method is presented for the efficient construction and delivery of complex genetic circuits into mammalian cells. Multi-gene circuits are assembled with high efficiency from a validated modular library into single pieces. The assembled circuits can be used for transient expression and each individual circuit can be integrated into a cellular genome to create a stable cell line. Genetic circuits were constructed that contain several expression units, including inducible control units and fluorescent markers. These circuits were delivered into Human Embryonic Kidney 293 (HEK293) cells for both transient and stable expression cases. Results show that the introduced genetic circuits performed as designed and that stable cell lines, each with the desired phenotype could be created efficiently. Several factors affecting the assembly efficiency and the performance of resulting circuits are also discussed. by Yinqing Li. S.M. 2012-07-02T15:48:14Z 2012-07-02T15:48:14Z 2011 2012 Thesis http://hdl.handle.net/1721.1/71499 796436655 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 75 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Li, Yinqing, Ph. D. Massachusetts Institute of Technology A novel method for mammalian large genetic circuit assembly and delivery |
| title | A novel method for mammalian large genetic circuit assembly and delivery |
| title_full | A novel method for mammalian large genetic circuit assembly and delivery |
| title_fullStr | A novel method for mammalian large genetic circuit assembly and delivery |
| title_full_unstemmed | A novel method for mammalian large genetic circuit assembly and delivery |
| title_short | A novel method for mammalian large genetic circuit assembly and delivery |
| title_sort | novel method for mammalian large genetic circuit assembly and delivery |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/71499 |
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