RNA sequencing reveals two major classes of gene expression levels in metazoan cells
The expression level of a gene is often used as a proxy for determining whether the protein or RNA product is functional in a cell or tissue. Therefore, it is of fundamental importance to understand the global distribution of gene expression levels, and to be able to interpret it mechanistically...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/71571 |
| _version_ | 1826211448408244224 |
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| author | Hebenstreit, Daniel Fang, Miaoqing Charoensawan, Varodom van Oudenaarden, Alexander Teichmann, Sarah A. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hebenstreit, Daniel Fang, Miaoqing Charoensawan, Varodom van Oudenaarden, Alexander Teichmann, Sarah A. |
| author_sort | Hebenstreit, Daniel |
| collection | MIT |
| description | The expression level of a gene is often used as a proxy for determining whether the
protein or RNA product is functional in a cell or tissue. Therefore, it is of fundamental
importance to understand the global distribution of gene expression levels, and to be
able to interpret it mechanistically and functionally. Here we use RNA sequencing of
mouse Th2 cells, coupled with a range of other techniques, to show that all genes can
be separated, based on their expression abundance, into two distinct groups: one
group comprising of lowly expressed and putatively non-functional mRNAs, and the
other of highly expressed mRNAs with active chromatin marks at their promoters. |
| first_indexed | 2024-09-23T15:06:02Z |
| format | Article |
| id | mit-1721.1/71571 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:06:02Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/715712022-09-29T12:40:48Z RNA sequencing reveals two major classes of gene expression levels in metazoan cells Hebenstreit, Daniel Fang, Miaoqing Charoensawan, Varodom van Oudenaarden, Alexander Teichmann, Sarah A. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics van Oudenaarden, Alexander Fang, Miaoqing van Oudenaarden, Alexander The expression level of a gene is often used as a proxy for determining whether the protein or RNA product is functional in a cell or tissue. Therefore, it is of fundamental importance to understand the global distribution of gene expression levels, and to be able to interpret it mechanistically and functionally. Here we use RNA sequencing of mouse Th2 cells, coupled with a range of other techniques, to show that all genes can be separated, based on their expression abundance, into two distinct groups: one group comprising of lowly expressed and putatively non-functional mRNAs, and the other of highly expressed mRNAs with active chromatin marks at their promoters. 2012-07-11T12:45:05Z 2012-07-11T12:45:05Z 2011-06 2011-03 Article http://purl.org/eprint/type/JournalArticle 1744-4292 http://hdl.handle.net/1721.1/71571 Hebenstreit, Daniel et al. “RNA sequencing reveals two major classes of gene expression levels in metazoan cells.” Molecular Systems Biology 7 (2011) en_US http://dx.doi.org/10.1038/msb.2011.28 Molecular Systems Biology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group Prof. van Oudenaarden via Mat Willmott |
| spellingShingle | Hebenstreit, Daniel Fang, Miaoqing Charoensawan, Varodom van Oudenaarden, Alexander Teichmann, Sarah A. RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| title | RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| title_full | RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| title_fullStr | RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| title_full_unstemmed | RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| title_short | RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
| title_sort | rna sequencing reveals two major classes of gene expression levels in metazoan cells |
| url | http://hdl.handle.net/1721.1/71571 |
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