Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

Background: Recombinant expression and purification of metallo-enzymes, including hydrogenases, at high-yields is challenging due to complex, and enzyme specific, post-translational maturation processes. Low fidelities of maturation result in preparations containing a significant fraction of inac...

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Bibliographic Details
Main Authors: Yacoby, Iftach, Tegler, Lotta Tollstoy, Pochekailov, Sergii, Zhang, Shuguang, King, Paul W.
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Public Library of Science 2012
Online Access:http://hdl.handle.net/1721.1/71737

Internet

http://hdl.handle.net/1721.1/71737

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