Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes
The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-m...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/71761 https://orcid.org/0000-0002-7852-4328 https://orcid.org/0000-0002-2876-610X |
| _version_ | 1811093394762498048 |
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| author | Blitzblau, Hannah G. Chan, Clara Sophia Hochwagen, Andreas Bell, Stephen P |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Blitzblau, Hannah G. Chan, Clara Sophia Hochwagen, Andreas Bell, Stephen P |
| author_sort | Blitzblau, Hannah G. |
| collection | MIT |
| description | The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-meiotic S phase (meiS) and the assembly of meiotic chromosome axes, remain poorly defined. We have used genome-wide approaches in Saccharomyces cerevisiae to measure the kinetics of pre-meiotic DNA replication and to investigate the interdependencies between replication and axis formation. We found that replication initiation was delayed for a large number of origins in meiS compared to mitosis and that meiotic cells were far more sensitive to replication inhibition, most likely due to the starvation conditions required for meiotic induction. Moreover, replication initiation was delayed even in the absence of chromosome axes, indicating replication timing is independent of the process of axis assembly. Finally, we found that cells were able to install axis components and initiate recombination on unreplicated DNA. Thus, although pre-meiotic DNA replication and meiotic chromosome axis formation occur concurrently, they are not strictly coupled. The functional separation of these processes reveals a modular method of building meiotic chromosomes and predicts that any crosstalk between these modules must occur through superimposed regulatory mechanisms. |
| first_indexed | 2024-09-23T15:44:34Z |
| format | Article |
| id | mit-1721.1/71761 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:44:34Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/717612022-09-29T15:51:14Z Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes Blitzblau, Hannah G. Chan, Clara Sophia Hochwagen, Andreas Bell, Stephen P Massachusetts Institute of Technology. Department of Biology Bell, Stephen P. Blitzblau, Hannah G. Chan, Clara Sophia Bell, Stephen P. The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-meiotic S phase (meiS) and the assembly of meiotic chromosome axes, remain poorly defined. We have used genome-wide approaches in Saccharomyces cerevisiae to measure the kinetics of pre-meiotic DNA replication and to investigate the interdependencies between replication and axis formation. We found that replication initiation was delayed for a large number of origins in meiS compared to mitosis and that meiotic cells were far more sensitive to replication inhibition, most likely due to the starvation conditions required for meiotic induction. Moreover, replication initiation was delayed even in the absence of chromosome axes, indicating replication timing is independent of the process of axis assembly. Finally, we found that cells were able to install axis components and initiate recombination on unreplicated DNA. Thus, although pre-meiotic DNA replication and meiotic chromosome axis formation occur concurrently, they are not strictly coupled. The functional separation of these processes reveals a modular method of building meiotic chromosomes and predicts that any crosstalk between these modules must occur through superimposed regulatory mechanisms. Howard Hughes Medical Institute. Predoctoral Fellowship 2012-07-23T18:41:31Z 2012-07-23T18:41:31Z 2012-05 2011-12 Article http://purl.org/eprint/type/JournalArticle 1553-7390 1553-7404 http://hdl.handle.net/1721.1/71761 Blitzblau, Hannah G. et al. “Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes.” Ed. R. Scott Hawley. PLoS Genetics 8.5 (2012): e1002643. https://orcid.org/0000-0002-7852-4328 https://orcid.org/0000-0002-2876-610X en_US http://dx.doi.org/10.1371/journal.pgen.1002643 PLoS Genetics Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Blitzblau, Hannah G. Chan, Clara Sophia Hochwagen, Andreas Bell, Stephen P Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes |
| title | Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes |
| title_full | Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes |
| title_fullStr | Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes |
| title_full_unstemmed | Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes |
| title_short | Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes |
| title_sort | separation of dna replication from the assembly of break competent meiotic chromosomes |
| url | http://hdl.handle.net/1721.1/71761 https://orcid.org/0000-0002-7852-4328 https://orcid.org/0000-0002-2876-610X |
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