Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs)
Cell-based biosensors (CBBs) are becoming an important tool for biosecurity applications and rapid diagnostics. For current CBBs technology, cell immobilization and high throughput fabrication are the main challenges. To address these in this study, the feasibility of bioprinting cell-laden hydrogel...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Institute of Electrical and Electronics Engineers (IEEE)
2012
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| Online Access: | http://hdl.handle.net/1721.1/71872 |
| _version_ | 1826202425172688896 |
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| author | Xu, F. Moon, S. Emre, Ahmet E. Lien, C. Turali, E. S. Demirci, Utkan |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Xu, F. Moon, S. Emre, Ahmet E. Lien, C. Turali, E. S. Demirci, Utkan |
| author_sort | Xu, F. |
| collection | MIT |
| description | Cell-based biosensors (CBBs) are becoming an important tool for biosecurity applications and rapid diagnostics. For current CBBs technology, cell immobilization and high throughput fabrication are the main challenges. To address these in this study, the feasibility of bioprinting cell-laden hydrogel to fabricate CBBs at high throughput was investigated and cell response was tracked by using lensless charge-coupled device (CCD) technology. This study indicated that (i) a cell-laden collagen printing platform was capable of immobilizing cells (smooth muscle cells) in collagen droplets with precise spatial control and pattern them onto surfaces, (ii) high post-printing cell viability was achieved (>94%) and the immobilized cells proliferated over five days, (iii) the immobilized cells maintain their biological and physiological sensitivity to environmental stimuli (e.g. environmental temperature change and lysis by adding of de-ionized water), as quantified by change in cell spread size (decreasing from ~3000 ¿m[superscript 2] at t = 0 hour to ~600 ¿m[superscript 2] at t = 16 hours), and (iv) our developed lensless CCD technology is capable detecting the cell morphology change under environmental stimuli, which is essential for the portability of the CBBs. These results show that printing cells encapsulated inbiocompatible hydrogels could lead to fabrication of CBBs in a high throughput manner. Also, the lensless CCD systems can be used to monitor the morphological cell responses over a wide field of view (as large as 37.25 mm à 25.70 mm). |
| first_indexed | 2024-09-23T12:07:16Z |
| format | Article |
| id | mit-1721.1/71872 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:07:16Z |
| publishDate | 2012 |
| publisher | Institute of Electrical and Electronics Engineers (IEEE) |
| record_format | dspace |
| spelling | mit-1721.1/718722022-09-28T00:16:48Z Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) Xu, F. Moon, S. Emre, Ahmet E. Lien, C. Turali, E. S. Demirci, Utkan Harvard University--MIT Division of Health Sciences and Technology Demirci, Utkan Demirci, Utkan Cell-based biosensors (CBBs) are becoming an important tool for biosecurity applications and rapid diagnostics. For current CBBs technology, cell immobilization and high throughput fabrication are the main challenges. To address these in this study, the feasibility of bioprinting cell-laden hydrogel to fabricate CBBs at high throughput was investigated and cell response was tracked by using lensless charge-coupled device (CCD) technology. This study indicated that (i) a cell-laden collagen printing platform was capable of immobilizing cells (smooth muscle cells) in collagen droplets with precise spatial control and pattern them onto surfaces, (ii) high post-printing cell viability was achieved (>94%) and the immobilized cells proliferated over five days, (iii) the immobilized cells maintain their biological and physiological sensitivity to environmental stimuli (e.g. environmental temperature change and lysis by adding of de-ionized water), as quantified by change in cell spread size (decreasing from ~3000 ¿m[superscript 2] at t = 0 hour to ~600 ¿m[superscript 2] at t = 16 hours), and (iv) our developed lensless CCD technology is capable detecting the cell morphology change under environmental stimuli, which is essential for the portability of the CBBs. These results show that printing cells encapsulated inbiocompatible hydrogels could lead to fabrication of CBBs in a high throughput manner. Also, the lensless CCD systems can be used to monitor the morphological cell responses over a wide field of view (as large as 37.25 mm à 25.70 mm). 2012-07-27T15:47:45Z 2012-07-27T15:47:45Z 2010-01 2009-10 Article http://purl.org/eprint/type/ConferencePaper 978-1-4244-5335-1 978-1-4244-4548-6 1930-0395 http://hdl.handle.net/1721.1/71872 Xu, F. et al. “Cell Bioprinting as a Potential High-throughput Method for Fabricating Cell-based Biosensors (CBBs).” IEEE, 2009. 387–391. © Copyright 2009 IEEE en_US http://dx.doi.org/10.1109/ICSENS.2009.5398245 2009 IEEE Sensors Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Institute of Electrical and Electronics Engineers (IEEE) IEEE |
| spellingShingle | Xu, F. Moon, S. Emre, Ahmet E. Lien, C. Turali, E. S. Demirci, Utkan Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) |
| title | Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) |
| title_full | Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) |
| title_fullStr | Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) |
| title_full_unstemmed | Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) |
| title_short | Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs) |
| title_sort | cell bioprinting as a potential high throughput method for fabricating cell based biosensors cbbs |
| url | http://hdl.handle.net/1721.1/71872 |
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