Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity
Resistance of tumor cells to platinum anticancer agents poses a major problem in cancer chemotherapy. One of the mechanisms associated with platinum-based drug resistance is the enhanced capacity of the cell to carry out nucleotide excision repair (NER) on platinum-damaged DNA. Endonucleases XPF and...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell (John Wiley & Sons)
2012
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| Online Access: | http://hdl.handle.net/1721.1/71926 https://orcid.org/0000-0002-2693-4982 |
| _version_ | 1826208077818363904 |
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| author | Graf, Nora Ang, Wee Han Zhu, Guangyu Myint, MyatNoeZin Lippard, Stephen J. |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Graf, Nora Ang, Wee Han Zhu, Guangyu Myint, MyatNoeZin Lippard, Stephen J. |
| author_sort | Graf, Nora |
| collection | MIT |
| description | Resistance of tumor cells to platinum anticancer agents poses a major problem in cancer chemotherapy. One of the mechanisms associated with platinum-based drug resistance is the enhanced capacity of the cell to carry out nucleotide excision repair (NER) on platinum-damaged DNA. Endonucleases XPF and XPG are critical components of NER, responsible for excising the damaged DNA strand to remove the DNA lesion. Here, we investigated possible consequences of down-regulation of XPF and XPG gene expression in osteosarcoma cancer cells (U2OS) and the impact on cellular transcription and DNA repair. We further evaluated the sensitivity of such cells toward the platinum anticancer drugs cisplatin and oxaliplatin. |
| first_indexed | 2024-09-23T14:00:06Z |
| format | Article |
| id | mit-1721.1/71926 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:00:06Z |
| publishDate | 2012 |
| publisher | Wiley Blackwell (John Wiley & Sons) |
| record_format | dspace |
| spelling | mit-1721.1/719262022-10-01T18:27:45Z Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity Graf, Nora Ang, Wee Han Zhu, Guangyu Myint, MyatNoeZin Lippard, Stephen J. Massachusetts Institute of Technology. Department of Chemistry Lippard, Stephen J. Graf, Nora Ang, Wee Han Zhu, Guangyu Myint, MyatNoeZin Lippard, Stephen J. Resistance of tumor cells to platinum anticancer agents poses a major problem in cancer chemotherapy. One of the mechanisms associated with platinum-based drug resistance is the enhanced capacity of the cell to carry out nucleotide excision repair (NER) on platinum-damaged DNA. Endonucleases XPF and XPG are critical components of NER, responsible for excising the damaged DNA strand to remove the DNA lesion. Here, we investigated possible consequences of down-regulation of XPF and XPG gene expression in osteosarcoma cancer cells (U2OS) and the impact on cellular transcription and DNA repair. We further evaluated the sensitivity of such cells toward the platinum anticancer drugs cisplatin and oxaliplatin. National Cancer Institute (U.S.) (Grant Number CA034992.) National University of Singapore. German Academic Exchange Service (DAAD) 2012-07-31T20:25:00Z 2012-07-31T20:25:00Z 2011-03 Article http://purl.org/eprint/type/JournalArticle 1439-4227 1439-7633 http://hdl.handle.net/1721.1/71926 Graf, Nora et al. “Role of Endonucleases XPF and XPG in Nucleotide Excision Repair of Platinated DNA and Cisplatin/Oxaliplatin Cytotoxicity.” ChemBioChem 12.7 (2011): 1115–1123. https://orcid.org/0000-0002-2693-4982 en_US http://dx.doi.org/10.1002/cbic.201000724 ChemBioChem Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Wiley Blackwell (John Wiley & Sons) PMC |
| spellingShingle | Graf, Nora Ang, Wee Han Zhu, Guangyu Myint, MyatNoeZin Lippard, Stephen J. Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity |
| title | Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity |
| title_full | Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity |
| title_fullStr | Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity |
| title_full_unstemmed | Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity |
| title_short | Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity |
| title_sort | role of endonucleases xpf and xpg in nucleotide excision repair of platinated dna and cisplatin oxaliplatin cytotoxicity |
| url | http://hdl.handle.net/1721.1/71926 https://orcid.org/0000-0002-2693-4982 |
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