High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 µm axial and < 2 µm transver...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Optical Society of America
2012
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| Online Access: | http://hdl.handle.net/1721.1/71933 https://orcid.org/0000-0002-0828-4357 |
| _version_ | 1826199072549109760 |
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| author | Aguirre, Aaron Dominic Sawinski, Juergen Huang, Shu-Wei Zhou, Chao Denk, Winfried Fujimoto, James G. |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Aguirre, Aaron Dominic Sawinski, Juergen Huang, Shu-Wei Zhou, Chao Denk, Winfried Fujimoto, James G. |
| author_sort | Aguirre, Aaron Dominic |
| collection | MIT |
| description | Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 µm axial and < 2 µm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications. |
| first_indexed | 2024-09-23T11:14:17Z |
| format | Article |
| id | mit-1721.1/71933 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:14:17Z |
| publishDate | 2012 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | mit-1721.1/719332022-10-01T02:16:17Z High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe Aguirre, Aaron Dominic Sawinski, Juergen Huang, Shu-Wei Zhou, Chao Denk, Winfried Fujimoto, James G. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Fujimoto, James G. Aguirre, Aaron Dominic Huang, Shu-Wei Zhou, Chao Fujimoto, James G. Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 µm axial and < 2 µm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications. National Institutes of Health (U.S.) (R01-CA75289-13) National Institutes of Health (U.S.) R01-EY11289-25 United States. Air Force Office of Scientific Research (FA9550-07-1-0101) United States. Air Force Office of Scientific Research (FA9550-07-1-0014) Max Planck Society for the Advancement of Science National Institutes of Health (U.S.) (Fellowship) (F31 EB005978) 2012-08-01T16:52:32Z 2012-08-01T16:52:32Z 2010-02 2010-02 Article http://purl.org/eprint/type/JournalArticle 1094-4087 http://hdl.handle.net/1721.1/71933 Aguirre, Aaron D. et al. “High Speed Optical Coherence Microscopy with Autofocus Adjustment and a Miniaturized Endoscopic Imaging Probe.” Optics Express 18.5 (2010): 4222. https://orcid.org/0000-0002-0828-4357 en_US http://dx.doi.org/10.1364/OE.18.004222 Optics Express Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Optical Society of America PubMed Central |
| spellingShingle | Aguirre, Aaron Dominic Sawinski, Juergen Huang, Shu-Wei Zhou, Chao Denk, Winfried Fujimoto, James G. High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| title | High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| title_full | High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| title_fullStr | High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| title_full_unstemmed | High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| title_short | High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| title_sort | high speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe |
| url | http://hdl.handle.net/1721.1/71933 https://orcid.org/0000-0002-0828-4357 |
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