Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents
Here we present a new bifunctional layer-by-layer (LbL) construct made by combining a permanent microbicidal polyelectrolyte multilayered (PEM) base film with a hydrolytically degradable PEM top film that offers controlled and localized delivery of therapeutics. Two degradable film architectures are...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2012
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| Online Access: | http://hdl.handle.net/1721.1/71936 https://orcid.org/0000-0003-3830-714X |
| _version_ | 1826207060955496448 |
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| author | Wong, Sze Yinn Moskowitz, Joshua Seth Veselinovic, Jovana Rosario, Ryan A. Timachova, Ksenia Blaisse, Michael R. Fuller, Renee C. Klibanov, Alexander M. Hammond, Paula T |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Wong, Sze Yinn Moskowitz, Joshua Seth Veselinovic, Jovana Rosario, Ryan A. Timachova, Ksenia Blaisse, Michael R. Fuller, Renee C. Klibanov, Alexander M. Hammond, Paula T |
| author_sort | Wong, Sze Yinn |
| collection | MIT |
| description | Here we present a new bifunctional layer-by-layer (LbL) construct made by combining a permanent microbicidal polyelectrolyte multilayered (PEM) base film with a hydrolytically degradable PEM top film that offers controlled and localized delivery of therapeutics. Two degradable film architectures are presented: (1) bolus release of an antibiotic (gentamicin) to eradicate initial infection at the implant site, or (2) sustained delivery of an anti-inflammatory drug (diclofenac) to cope with inflammation at the site of implantation due to tissue injury. Each degradable film was built on top of a permanent base film that imparts the implantable device surface with microbicidal functionality that prevents the formation of biofilms. Controlled-delivery of gentamicin was demonstrated over hours and that of diclofenac over days. Both drugs retained their efficacy upon release. The permanent microbicidal base film was biocompatible with A549 epithelial cancer cells and MC3T3-E1 osteoprogenitor cells, while also preventing bacteria attachment from turbid media for the entire duration of the two weeks studied. The microbicidal base film retains its functionality after the biodegradable films have completely degraded. The versatility of these PEM films and their ability to prevent biofilm formation make them attractive as coatings for implantable devices. |
| first_indexed | 2024-09-23T13:43:34Z |
| format | Article |
| id | mit-1721.1/71936 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:43:34Z |
| publishDate | 2012 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/719362022-10-01T16:44:00Z Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents Wong, Sze Yinn Moskowitz, Joshua Seth Veselinovic, Jovana Rosario, Ryan A. Timachova, Ksenia Blaisse, Michael R. Fuller, Renee C. Klibanov, Alexander M. Hammond, Paula T Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Klibanov, Alexander M. Wong, Sze Yinn Moskowitz, Joshua Seth Veselinovic, Jovana Rosario, Ryan A. Timachova, Ksenia Blaisse, Michael R. Fuller, Renee C. Klibanov, Alexander M. Hammond, Paula T. Here we present a new bifunctional layer-by-layer (LbL) construct made by combining a permanent microbicidal polyelectrolyte multilayered (PEM) base film with a hydrolytically degradable PEM top film that offers controlled and localized delivery of therapeutics. Two degradable film architectures are presented: (1) bolus release of an antibiotic (gentamicin) to eradicate initial infection at the implant site, or (2) sustained delivery of an anti-inflammatory drug (diclofenac) to cope with inflammation at the site of implantation due to tissue injury. Each degradable film was built on top of a permanent base film that imparts the implantable device surface with microbicidal functionality that prevents the formation of biofilms. Controlled-delivery of gentamicin was demonstrated over hours and that of diclofenac over days. Both drugs retained their efficacy upon release. The permanent microbicidal base film was biocompatible with A549 epithelial cancer cells and MC3T3-E1 osteoprogenitor cells, while also preventing bacteria attachment from turbid media for the entire duration of the two weeks studied. The microbicidal base film retains its functionality after the biodegradable films have completely degraded. The versatility of these PEM films and their ability to prevent biofilm formation make them attractive as coatings for implantable devices. United States. Army Research Office. Institute for Soldier Nanotechnologies National Institutes of Health (U.S.) National Institute on Aging (5R01AG029601-03) 2012-08-01T18:14:45Z 2012-08-01T18:14:45Z 2010-11 2010-07 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/71936 Wong, Sze Yinn et al. “Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents.” Journal of the American Chemical Society 132.50 (2010): 17840–17848. https://orcid.org/0000-0003-3830-714X en_US http://dx.doi.org/10.1021/ja106288c Journal of the American Chemical Society Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Wong, Sze Yinn Moskowitz, Joshua Seth Veselinovic, Jovana Rosario, Ryan A. Timachova, Ksenia Blaisse, Michael R. Fuller, Renee C. Klibanov, Alexander M. Hammond, Paula T Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents |
| title | Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents |
| title_full | Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents |
| title_fullStr | Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents |
| title_full_unstemmed | Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents |
| title_short | Dual Functional Polyelectrolyte Multilayer Coatings for Implants: Permanent Microbicidal Base with Controlled Release of Therapeutic Agents |
| title_sort | dual functional polyelectrolyte multilayer coatings for implants permanent microbicidal base with controlled release of therapeutic agents |
| url | http://hdl.handle.net/1721.1/71936 https://orcid.org/0000-0003-3830-714X |
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