Subunit architecture of general transcription factor TFIIH
Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the “minimal core” subunits (Ssl1, Tfb1, Tfb2,...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences
2012
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| Online Access: | http://hdl.handle.net/1721.1/72183 |
| _version_ | 1811091187032915968 |
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| author | Brignole, Edward J. Gibbons, Brian J. Azubel, Maia Murakami, Kenji Voss, Neil R. Bushnell, David A. Asturias, Francisco J. Kornberg, Roger D. |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Brignole, Edward J. Gibbons, Brian J. Azubel, Maia Murakami, Kenji Voss, Neil R. Bushnell, David A. Asturias, Francisco J. Kornberg, Roger D. |
| author_sort | Brignole, Edward J. |
| collection | MIT |
| description | Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the “minimal core” subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer. These results can be further interpreted by placement of previous X-ray structures into the additional densities to give a preliminary picture of the RNA polymerase II preinitiation complex. In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain. |
| first_indexed | 2024-09-23T14:58:19Z |
| format | Article |
| id | mit-1721.1/72183 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:58:19Z |
| publishDate | 2012 |
| publisher | National Academy of Sciences |
| record_format | dspace |
| spelling | mit-1721.1/721832022-10-01T23:39:39Z Subunit architecture of general transcription factor TFIIH Brignole, Edward J. Gibbons, Brian J. Azubel, Maia Murakami, Kenji Voss, Neil R. Bushnell, David A. Asturias, Francisco J. Kornberg, Roger D. Massachusetts Institute of Technology. Department of Chemistry Brignole, Edward J. Brignole, Edward J. Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the “minimal core” subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer. These results can be further interpreted by placement of previous X-ray structures into the additional densities to give a preliminary picture of the RNA polymerase II preinitiation complex. In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain. National Cancer Institute (U.S.) (Grant number F32DK080622) 2012-08-17T14:47:53Z 2012-08-17T14:47:53Z 2012-01 2011-04 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/72183 Gibbons, B. J. et al. “Subunit Architecture of General Transcription Factor TFIIH.” Proceedings of the National Academy of Sciences 109.6 (2012): 1949–1954. Copyright ©2012 by the National Academy of Sciences en_US http://dx.doi.org/10.1073/pnas.1105266109 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences PNAS |
| spellingShingle | Brignole, Edward J. Gibbons, Brian J. Azubel, Maia Murakami, Kenji Voss, Neil R. Bushnell, David A. Asturias, Francisco J. Kornberg, Roger D. Subunit architecture of general transcription factor TFIIH |
| title | Subunit architecture of general transcription factor TFIIH |
| title_full | Subunit architecture of general transcription factor TFIIH |
| title_fullStr | Subunit architecture of general transcription factor TFIIH |
| title_full_unstemmed | Subunit architecture of general transcription factor TFIIH |
| title_short | Subunit architecture of general transcription factor TFIIH |
| title_sort | subunit architecture of general transcription factor tfiih |
| url | http://hdl.handle.net/1721.1/72183 |
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