DNA-CNT Nanowire Networks for DNA Detection
The ability to detect biological analytes in a rapid, sensitive, operationally simple, and cost-effective manner will impact human health and safety. Hybrid biocatalyzed-carbon nanotube (CNT) nanowire-based detection methods offer a highly sensitive and specific platform for the fabrication of simpl...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2012
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| Online Access: | http://hdl.handle.net/1721.1/72193 |
| _version_ | 1811092934833995776 |
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| author | Weizmann, Yossi Chenoweth, David M. Swager, Timothy M |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Weizmann, Yossi Chenoweth, David M. Swager, Timothy M |
| author_sort | Weizmann, Yossi |
| collection | MIT |
| description | The ability to detect biological analytes in a rapid, sensitive, operationally simple, and cost-effective manner will impact human health and safety. Hybrid biocatalyzed-carbon nanotube (CNT) nanowire-based detection methods offer a highly sensitive and specific platform for the fabrication of simple and effective conductometric devices. Here, we report a conductivity-based DNA detection method utilizing carbon nanotube−DNA nanowire devices and oligonucleotide-functionalized enzyme probes. Key to our sensor design is a DNA-linked-CNT wire motif, which forms a network of interrupted carbon nanotube wires connecting two electrodes. Sensing occurs at the DNA junctions linking CNTs, followed by amplification using enzymatic metalization leading to a conductimetric response. The DNA analyte detection limit is 10 fM with the ability to discriminate single, double, and triple base pair mismatches. DNA−CNT nanowires and device sensing gaps were characterized by scanning electron microscopy (SEM) and confocal Raman microscopy, supporting the enhanced conductometric response resulting from nanowire metallization. |
| first_indexed | 2024-09-23T15:36:00Z |
| format | Article |
| id | mit-1721.1/72193 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:36:00Z |
| publishDate | 2012 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/721932022-09-29T14:57:55Z DNA-CNT Nanowire Networks for DNA Detection Weizmann, Yossi Chenoweth, David M. Swager, Timothy M Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Massachusetts Institute of Technology. Department of Chemistry Swager, Timothy Manning Weizmann, Yossi Chenoweth, David M. Swager, Timothy Manning The ability to detect biological analytes in a rapid, sensitive, operationally simple, and cost-effective manner will impact human health and safety. Hybrid biocatalyzed-carbon nanotube (CNT) nanowire-based detection methods offer a highly sensitive and specific platform for the fabrication of simple and effective conductometric devices. Here, we report a conductivity-based DNA detection method utilizing carbon nanotube−DNA nanowire devices and oligonucleotide-functionalized enzyme probes. Key to our sensor design is a DNA-linked-CNT wire motif, which forms a network of interrupted carbon nanotube wires connecting two electrodes. Sensing occurs at the DNA junctions linking CNTs, followed by amplification using enzymatic metalization leading to a conductimetric response. The DNA analyte detection limit is 10 fM with the ability to discriminate single, double, and triple base pair mismatches. DNA−CNT nanowires and device sensing gaps were characterized by scanning electron microscopy (SEM) and confocal Raman microscopy, supporting the enhanced conductometric response resulting from nanowire metallization. United States. Army Research Office (W911NF-07-D-0004) National Institute of General Medical Sciences (U.S.). Postdoctural Fellowship (1-F32-GM087028-01A1) 2012-08-17T18:09:31Z 2012-08-17T18:09:31Z 2011-02 2010-10 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/72193 Weizmann, Yossi, David M. Chenoweth, and Timothy M. Swager. “DNA−CNT Nanowire Networks for DNA Detection.” Journal of the American Chemical Society 133.10 (2011): 3238–3241. en_US http://dx.doi.org/10.1021/ja109180d Journal of the American Chemical Society Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Weizmann, Yossi Chenoweth, David M. Swager, Timothy M DNA-CNT Nanowire Networks for DNA Detection |
| title | DNA-CNT Nanowire Networks for DNA Detection |
| title_full | DNA-CNT Nanowire Networks for DNA Detection |
| title_fullStr | DNA-CNT Nanowire Networks for DNA Detection |
| title_full_unstemmed | DNA-CNT Nanowire Networks for DNA Detection |
| title_short | DNA-CNT Nanowire Networks for DNA Detection |
| title_sort | dna cnt nanowire networks for dna detection |
| url | http://hdl.handle.net/1721.1/72193 |
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