Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip
In this paper, we report a novel method for fabricating ion-selective membranes in poly(dimethylsiloxane) (PDMS)/glass-based microfluidic preconcentrators. Based on the concept of capillary valves, this fabrication method involves filling a lithographically patterned junction between two microchanne...
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry (RSC Publishing)
2012
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| Online Access: | http://hdl.handle.net/1721.1/72360 https://orcid.org/0000-0003-0834-5823 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826198757403787264 |
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| author | Liu, Vincent Hok Song, Yong-Ak Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Liu, Vincent Hok Song, Yong-Ak Han, Jongyoon |
| author_sort | Liu, Vincent Hok |
| collection | MIT |
| description | In this paper, we report a novel method for fabricating ion-selective membranes in poly(dimethylsiloxane) (PDMS)/glass-based microfluidic preconcentrators. Based on the concept of capillary valves, this fabrication method involves filling a lithographically patterned junction between two microchannels with an ion-selective material such as Nafion resin; subsequent curing results in a high aspect-ratio membrane for use in electrokinetic sample preconcentration. To demonstrate the concentration performance of this high-aspect-ratio, ion-selective membrane, we integrated the preconcentrator with a surface-based immunoassay for R-Phycoerythrin (RPE). Using a 1× PBS buffer system, the preconcentrator-enhanced immunoassay showed an approximately 100× improvement in sensitivity within 30 min. This is the first time that an electrokinetic microfluidic preconcentrator based on ion concentration polarization (ICP) has been used in high ionic strength buffer solutions to enhance the sensitivity of a surface-based immunoassay. |
| first_indexed | 2024-09-23T11:09:37Z |
| format | Article |
| id | mit-1721.1/72360 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:09:37Z |
| publishDate | 2012 |
| publisher | Royal Society of Chemistry (RSC Publishing) |
| record_format | dspace |
| spelling | mit-1721.1/723602022-10-01T01:40:41Z Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip Liu, Vincent Hok Song, Yong-Ak Han, Jongyoon Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Han, Jongyoon Liu, Vincent Hok Song, Yong-Ak Han, Jongyoon In this paper, we report a novel method for fabricating ion-selective membranes in poly(dimethylsiloxane) (PDMS)/glass-based microfluidic preconcentrators. Based on the concept of capillary valves, this fabrication method involves filling a lithographically patterned junction between two microchannels with an ion-selective material such as Nafion resin; subsequent curing results in a high aspect-ratio membrane for use in electrokinetic sample preconcentration. To demonstrate the concentration performance of this high-aspect-ratio, ion-selective membrane, we integrated the preconcentrator with a surface-based immunoassay for R-Phycoerythrin (RPE). Using a 1× PBS buffer system, the preconcentrator-enhanced immunoassay showed an approximately 100× improvement in sensitivity within 30 min. This is the first time that an electrokinetic microfluidic preconcentrator based on ion concentration polarization (ICP) has been used in high ionic strength buffer solutions to enhance the sensitivity of a surface-based immunoassay. National Institutes of Health (U.S.) (CA119402) National Institutes of Health (U.S.) (EB005743) 2012-08-28T12:58:00Z 2012-08-28T12:58:00Z 2010-02 2009-11 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/72360 Liu, Vincent, Yong-Ak Song, and Jongyoon Han. “Capillary-valve-based Fabrication of Ion-selective Membrane Junction for Electrokinetic Sample Preconcentration in PDMS Chip.” Lab on a Chip 10.11 (2010): 1485. https://orcid.org/0000-0003-0834-5823 https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1039/b923214a Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Royal Society of Chemistry (RSC Publishing) PubMed Central |
| spellingShingle | Liu, Vincent Hok Song, Yong-Ak Han, Jongyoon Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip |
| title | Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip |
| title_full | Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip |
| title_fullStr | Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip |
| title_full_unstemmed | Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip |
| title_short | Capillary-valve-based fabrication of ion-selective membrane junction for electrokinetic sample preconcentration in PDMS chip |
| title_sort | capillary valve based fabrication of ion selective membrane junction for electrokinetic sample preconcentration in pdms chip |
| url | http://hdl.handle.net/1721.1/72360 https://orcid.org/0000-0003-0834-5823 https://orcid.org/0000-0001-7215-1439 |
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