Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation
Background: Local microbial infections induced by multiple-drug-resistant bacteria in the orthopedic field can be intractable, therefore development of new therapeutic modalities is needed. Photodynamic therapy (PDT) is a promising alternative modality to antibiotics for intractable microbial infec...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/72385 |
| _version_ | 1826189352893415424 |
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| author | Tanaka, Masamitsu Mroz, Pawel Dai, Tianhong Huang, Liyi Morimoto, Yuji Kinoshita, Manabu Yoshihara, Yasuo Nemoto, Koichi Shinomiya, Nariyoshi Seki, Suhji Hamblin, Michael R. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Tanaka, Masamitsu Mroz, Pawel Dai, Tianhong Huang, Liyi Morimoto, Yuji Kinoshita, Manabu Yoshihara, Yasuo Nemoto, Koichi Shinomiya, Nariyoshi Seki, Suhji Hamblin, Michael R. |
| author_sort | Tanaka, Masamitsu |
| collection | MIT |
| description | Background:
Local microbial infections induced by multiple-drug-resistant bacteria in the orthopedic field can be intractable, therefore development of new therapeutic modalities is needed. Photodynamic therapy (PDT) is a promising alternative modality to antibiotics for intractable microbial infections, and we recently reported that PDT has the potential to accumulate neutrophils into the infected site which leads to resolution of the infection. PDT for cancer has long been known to be able to stimulate the innate and adaptive arms of the immune system.
Methodology/Principal Findings:
In the present study, a murine methicillin-resistant Staphylococcus aureus (MRSA) arthritis model using bioluminescent MRSA and polystyrene microparticles was established, and both the therapeutic (Th-PDT) and preventive (Pre-PDT) effects of PDT using methylene blue as photosensitizer were examined. Although Th-PDT could not demonstrate direct bacterial killing, neutrophils were accumulated into the infectious joint space after PDT and MRSA arthritis was reduced. With the preconditioning Pre-PDT regimen, neutrophils were quickly accumulated into the joint immediately after bacterial inoculation and bacterial growth was suppressed and the establishment of infection was inhibited.
Conclusions/Significance:
This is the first demonstration of a protective innate immune response against a bacterial pathogen produced by PDT. |
| first_indexed | 2024-09-23T08:13:43Z |
| format | Article |
| id | mit-1721.1/72385 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:13:43Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/723852022-09-30T08:24:20Z Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation Tanaka, Masamitsu Mroz, Pawel Dai, Tianhong Huang, Liyi Morimoto, Yuji Kinoshita, Manabu Yoshihara, Yasuo Nemoto, Koichi Shinomiya, Nariyoshi Seki, Suhji Hamblin, Michael R. Harvard University--MIT Division of Health Sciences and Technology Hamblin, Michael R. Hamblin, Michael R. Background: Local microbial infections induced by multiple-drug-resistant bacteria in the orthopedic field can be intractable, therefore development of new therapeutic modalities is needed. Photodynamic therapy (PDT) is a promising alternative modality to antibiotics for intractable microbial infections, and we recently reported that PDT has the potential to accumulate neutrophils into the infected site which leads to resolution of the infection. PDT for cancer has long been known to be able to stimulate the innate and adaptive arms of the immune system. Methodology/Principal Findings: In the present study, a murine methicillin-resistant Staphylococcus aureus (MRSA) arthritis model using bioluminescent MRSA and polystyrene microparticles was established, and both the therapeutic (Th-PDT) and preventive (Pre-PDT) effects of PDT using methylene blue as photosensitizer were examined. Although Th-PDT could not demonstrate direct bacterial killing, neutrophils were accumulated into the infectious joint space after PDT and MRSA arthritis was reduced. With the preconditioning Pre-PDT regimen, neutrophils were quickly accumulated into the joint immediately after bacterial inoculation and bacterial growth was suppressed and the establishment of infection was inhibited. Conclusions/Significance: This is the first demonstration of a protective innate immune response against a bacterial pathogen produced by PDT. National Institutes of Health (U.S.) (Grant number R01AI050875) 2012-08-28T19:09:23Z 2012-08-28T19:09:23Z 2012-06 2012-05 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/72385 Tanaka, Masamitsu et al. “Photodynamic Therapy Can Induce a Protective Innate Immune Response Against Murine Bacterial Arthritis via Neutrophil Accumulation.” Ed. Hossam M. Ashour. PLoS ONE 7.6 (2012): e39823. en_US http://dx.doi.org/10.1371/journal.pone.0039823 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Tanaka, Masamitsu Mroz, Pawel Dai, Tianhong Huang, Liyi Morimoto, Yuji Kinoshita, Manabu Yoshihara, Yasuo Nemoto, Koichi Shinomiya, Nariyoshi Seki, Suhji Hamblin, Michael R. Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation |
| title | Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation |
| title_full | Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation |
| title_fullStr | Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation |
| title_full_unstemmed | Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation |
| title_short | Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation |
| title_sort | photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation |
| url | http://hdl.handle.net/1721.1/72385 |
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