Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes
Pharmacological studies suggest that dopamine release from lateral olivocochlear efferent neurons suppresses spontaneous and sound-evoked activity in cochlear nerve fibers and helps control noise-induced excitotoxicity; however, the literature on cochlear expression and localization of dopamine rece...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Society for Neuroscience
2012
|
| Online Access: | http://hdl.handle.net/1721.1/72562 |
| _version_ | 1826195050936139776 |
|---|---|
| author | Maison, Stephane F. Liu, Xiao-Ping Eatock, Ruth Anne Sibley, David R. Grandy, David K. Liberman, M. Charles |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Maison, Stephane F. Liu, Xiao-Ping Eatock, Ruth Anne Sibley, David R. Grandy, David K. Liberman, M. Charles |
| author_sort | Maison, Stephane F. |
| collection | MIT |
| description | Pharmacological studies suggest that dopamine release from lateral olivocochlear efferent neurons suppresses spontaneous and sound-evoked activity in cochlear nerve fibers and helps control noise-induced excitotoxicity; however, the literature on cochlear expression and localization of dopamine receptors is contradictory. To better characterize cochlear dopaminergic signaling, we studied receptor localization using immunohistochemistry or reverse transcriptase PCR and assessed histopathology, cochlear responses and olivocochlear function in mice with targeted deletion of each of the five receptor subtypes. In normal ears, D1, D2, and D5 receptors were detected in microdissected immature (postnatal days 10–13) spiral ganglion cells and outer hair cells but not inner hair cells. D4 was detected in spiral ganglion cells only. In whole cochlea samples from adults, transcripts for D1, D2, D4, and D5 were present, whereas D3 mRNA was never detected. D1 and D2 immunolabeling was localized to cochlear nerve fibers, near the first nodes of Ranvier (D2) and in the inner spiral bundle region (D1 and D2) where presynaptic olivocochlear terminals are found. No other receptor labeling was consistent. Cochlear function was normal in D3, D4, and D5 knock-outs. D1 and D2 knock-outs showed slight, but significant enhancement and suppression, respectively, of cochlear responses, both in the neural output [auditory brainstem response (ABR) wave 1] and in outer hair cell function [distortion product otoacoustic emissions (DPOAEs)]. Vulnerability to acoustic injury was significantly increased in D2, D4 and D5 lines: D1 could not be tested, and no differences were seen in D3 mutants, consistent with a lack of receptor expression. The increased vulnerability in D2 knock-outs was seen in DPOAEs, suggesting a role for dopamine in the outer hair cell area. In D4 and D5 knock-outs, the increased noise vulnerability was seen only in ABRs, consistent with a role for dopaminergic signaling in minimizing neural damage. |
| first_indexed | 2024-09-23T10:06:07Z |
| format | Article |
| id | mit-1721.1/72562 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:06:07Z |
| publishDate | 2012 |
| publisher | Society for Neuroscience |
| record_format | dspace |
| spelling | mit-1721.1/725622022-09-26T15:43:33Z Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes Maison, Stephane F. Liu, Xiao-Ping Eatock, Ruth Anne Sibley, David R. Grandy, David K. Liberman, M. Charles Harvard University--MIT Division of Health Sciences and Technology Liberman, M. Charles Liu, Xiao-Ping Liberman, M. Charles Pharmacological studies suggest that dopamine release from lateral olivocochlear efferent neurons suppresses spontaneous and sound-evoked activity in cochlear nerve fibers and helps control noise-induced excitotoxicity; however, the literature on cochlear expression and localization of dopamine receptors is contradictory. To better characterize cochlear dopaminergic signaling, we studied receptor localization using immunohistochemistry or reverse transcriptase PCR and assessed histopathology, cochlear responses and olivocochlear function in mice with targeted deletion of each of the five receptor subtypes. In normal ears, D1, D2, and D5 receptors were detected in microdissected immature (postnatal days 10–13) spiral ganglion cells and outer hair cells but not inner hair cells. D4 was detected in spiral ganglion cells only. In whole cochlea samples from adults, transcripts for D1, D2, D4, and D5 were present, whereas D3 mRNA was never detected. D1 and D2 immunolabeling was localized to cochlear nerve fibers, near the first nodes of Ranvier (D2) and in the inner spiral bundle region (D1 and D2) where presynaptic olivocochlear terminals are found. No other receptor labeling was consistent. Cochlear function was normal in D3, D4, and D5 knock-outs. D1 and D2 knock-outs showed slight, but significant enhancement and suppression, respectively, of cochlear responses, both in the neural output [auditory brainstem response (ABR) wave 1] and in outer hair cell function [distortion product otoacoustic emissions (DPOAEs)]. Vulnerability to acoustic injury was significantly increased in D2, D4 and D5 lines: D1 could not be tested, and no differences were seen in D3 mutants, consistent with a lack of receptor expression. The increased vulnerability in D2 knock-outs was seen in DPOAEs, suggesting a role for dopamine in the outer hair cell area. In D4 and D5 knock-outs, the increased noise vulnerability was seen only in ABRs, consistent with a role for dopaminergic signaling in minimizing neural damage. 2012-09-07T14:33:28Z 2012-09-07T14:33:28Z 2012-01 2011-10 Article http://purl.org/eprint/type/JournalArticle 0270-6474 1529-2401 http://hdl.handle.net/1721.1/72562 Maison, S. F. et al. “Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes.” Journal of Neuroscience 32.1 (2012): 344–355. en_US http://dx.doi.org/10.1523/jneurosci.4720-11.2012 Journal of Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society for Neuroscience SFN |
| spellingShingle | Maison, Stephane F. Liu, Xiao-Ping Eatock, Ruth Anne Sibley, David R. Grandy, David K. Liberman, M. Charles Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes |
| title | Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes |
| title_full | Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes |
| title_fullStr | Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes |
| title_full_unstemmed | Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes |
| title_short | Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes |
| title_sort | dopaminergic signaling in the cochlea receptor expression patterns and deletion phenotypes |
| url | http://hdl.handle.net/1721.1/72562 |
| work_keys_str_mv | AT maisonstephanef dopaminergicsignalinginthecochleareceptorexpressionpatternsanddeletionphenotypes AT liuxiaoping dopaminergicsignalinginthecochleareceptorexpressionpatternsanddeletionphenotypes AT eatockruthanne dopaminergicsignalinginthecochleareceptorexpressionpatternsanddeletionphenotypes AT sibleydavidr dopaminergicsignalinginthecochleareceptorexpressionpatternsanddeletionphenotypes AT grandydavidk dopaminergicsignalinginthecochleareceptorexpressionpatternsanddeletionphenotypes AT libermanmcharles dopaminergicsignalinginthecochleareceptorexpressionpatternsanddeletionphenotypes |