Metabolic cycling without cell division cycling in respiring yeast
Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multi...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
National Academy of Sciences (U.S.)
2012
|
| Online Access: | http://hdl.handle.net/1721.1/72990 https://orcid.org/0000-0003-2035-1820 |
| _version_ | 1826191835585839104 |
|---|---|
| author | Macinskas, Joanna Caudy, Amy Botstein, David Slavov, Nikolai G |
| author2 | MIT Materials Research Laboratory |
| author_facet | MIT Materials Research Laboratory Macinskas, Joanna Caudy, Amy Botstein, David Slavov, Nikolai G |
| author_sort | Macinskas, Joanna |
| collection | MIT |
| description | Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multiple metabolic cycles, whereas the fraction of cells in the G1/G0 phase of the CDC increases monotonically from 90 to 99%. This demonstrates that metabolic cycling does not require cell division cycling and that metabolic synchrony does not require carbon-source limitation. More than 3,000 genes, including most genes annotated to the CDC, were expressed periodically in our batch culture, albeit a mere 10% of the cells divided asynchronously; only a smaller subset of CDC genes correlated with cell division. These results suggest that the yeast metabolic cycle reflects a growth cycle during G1/G0 and explains our previous puzzling observation that genes annotated to the CDC increase in expression at slow growth. |
| first_indexed | 2024-09-23T09:02:01Z |
| format | Article |
| id | mit-1721.1/72990 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:02:01Z |
| publishDate | 2012 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/729902022-09-30T12:55:22Z Metabolic cycling without cell division cycling in respiring yeast Macinskas, Joanna Caudy, Amy Botstein, David Slavov, Nikolai G MIT Materials Research Laboratory Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics Slavov, Nikolai G. Slavov, Nikolai G. Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multiple metabolic cycles, whereas the fraction of cells in the G1/G0 phase of the CDC increases monotonically from 90 to 99%. This demonstrates that metabolic cycling does not require cell division cycling and that metabolic synchrony does not require carbon-source limitation. More than 3,000 genes, including most genes annotated to the CDC, were expressed periodically in our batch culture, albeit a mere 10% of the cells divided asynchronously; only a smaller subset of CDC genes correlated with cell division. These results suggest that the yeast metabolic cycle reflects a growth cycle during G1/G0 and explains our previous puzzling observation that genes annotated to the CDC increase in expression at slow growth. National Institutes of Health (U.S.) (NIH Grant GM046406) National Institute of General Medical Sciences (U.S.) (Grant Number GM071508) 2012-09-17T15:12:42Z 2012-09-17T15:12:42Z 2011-11 2011-09 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/72990 Slavov, N. et al. “Metabolic Cycling Without Cell Division Cycling in Respiring Yeast.” Proceedings of the National Academy of Sciences 108.47 (2011): 19090–19095. Web. https://orcid.org/0000-0003-2035-1820 en_US http://dx.doi.org/10.1073/pnas.1116998108 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Macinskas, Joanna Caudy, Amy Botstein, David Slavov, Nikolai G Metabolic cycling without cell division cycling in respiring yeast |
| title | Metabolic cycling without cell division cycling in respiring yeast |
| title_full | Metabolic cycling without cell division cycling in respiring yeast |
| title_fullStr | Metabolic cycling without cell division cycling in respiring yeast |
| title_full_unstemmed | Metabolic cycling without cell division cycling in respiring yeast |
| title_short | Metabolic cycling without cell division cycling in respiring yeast |
| title_sort | metabolic cycling without cell division cycling in respiring yeast |
| url | http://hdl.handle.net/1721.1/72990 https://orcid.org/0000-0003-2035-1820 |
| work_keys_str_mv | AT macinskasjoanna metaboliccyclingwithoutcelldivisioncyclinginrespiringyeast AT caudyamy metaboliccyclingwithoutcelldivisioncyclinginrespiringyeast AT botsteindavid metaboliccyclingwithoutcelldivisioncyclinginrespiringyeast AT slavovnikolaig metaboliccyclingwithoutcelldivisioncyclinginrespiringyeast |