Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis

Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis

We describe a large-scale random approach termed reduced representation bisulfite sequencing (RRBS) for analyzing and comparing genomic methylation patterns. BglII restriction fragments were size-selected to 500–600 bp, equipped with adapters, treated with bisulfite, PCR amplified, cloned and sequen...

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Bibliographic Details
Main Authors: Meissner, Alexander, Gnirke, Andreas, Bell, George W., Ramsahoye, Bernard, Jaenisch, Rudolf, Lander, Eric Steven
Other Authors: Broad Institute of MIT and Harvard
Format: Article
Language:en_US
Published: Oxford University Press 2012
Online Access:http://hdl.handle.net/1721.1/73016

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