Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo
Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically e...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences of the United States of America
2012
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| Online Access: | http://hdl.handle.net/1721.1/73091 https://orcid.org/0000-0003-2442-5671 |
| _version_ | 1826211488800440320 |
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| author | Mower, Amanda F. Yu, Hongbo Majewska, Ania K. Okamoto, Ken-Ichi Hayashi, Yasunori Sur, Mriganka Kwok, Show Ming |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Mower, Amanda F. Yu, Hongbo Majewska, Ania K. Okamoto, Ken-Ichi Hayashi, Yasunori Sur, Mriganka Kwok, Show Ming |
| author_sort | Mower, Amanda F. |
| collection | MIT |
| description | Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically engineered Förster resonance energy transfer (FRET) probe for the detection of CaMKII activity, and two-photon imaging of single synapses within identified functional domains, we have revealed unexpected and differential mechanisms in specific subsets of synapses in vivo. Brief monocular deprivation leads to activation of CaMKII in most synapses of layer 2/3 pyramidal cells within deprived eye domains, despite reduced visual drive, but not in nondeprived eye domains. Synapses that are eliminated in deprived eye domains have low basal CaMKII activity, implying a protective role for activated CaMKII against synapse elimination. |
| first_indexed | 2024-09-23T15:06:45Z |
| format | Article |
| id | mit-1721.1/73091 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:06:45Z |
| publishDate | 2012 |
| publisher | National Academy of Sciences of the United States of America |
| record_format | dspace |
| spelling | mit-1721.1/730912022-09-29T12:45:14Z Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo Mower, Amanda F. Yu, Hongbo Majewska, Ania K. Okamoto, Ken-Ichi Hayashi, Yasunori Sur, Mriganka Kwok, Show Ming Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory RIKEN-MIT Center for Neural Circuit Genetics Mower, Amanda F. Kwok, Showming Yu, Hongbo Majewska, Ania K. Okamoto, Ken-Ichi Hayashi, Yasunori Sur, Mriganka Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically engineered Förster resonance energy transfer (FRET) probe for the detection of CaMKII activity, and two-photon imaging of single synapses within identified functional domains, we have revealed unexpected and differential mechanisms in specific subsets of synapses in vivo. Brief monocular deprivation leads to activation of CaMKII in most synapses of layer 2/3 pyramidal cells within deprived eye domains, despite reduced visual drive, but not in nondeprived eye domains. Synapses that are eliminated in deprived eye domains have low basal CaMKII activity, implying a protective role for activated CaMKII against synapse elimination. National Basic Research Program of China (973 Program) (2010CB327901) Japan. Ministry of Education, Culture, Sports, Science and Technology. Foundation of Synapse and Neurocircuit Pathology. (Grant-in-Aid for Scientific Research on Innovative Area) RIKEN Brain Science Institute National Institutes of Health (U.S.) (Grant R01DA17310) National Institutes of Health (U.S.) (Postdoctoral Fellowship) Japan Society for the Promotion of Science (Grant-In-Aid for Scientific Research) 2012-09-20T20:51:13Z 2012-09-20T20:51:13Z 2011-12 2011-05 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/73091 Mower, A. F. et al. “Experience-dependent Regulation of CaMKII Activity Within Single Visual Cortex Synapses in Vivo.” Proceedings of the National Academy of Sciences 108.52 (2011): 21241–21246. Copyright ©2011 by the National Academy of Sciences https://orcid.org/0000-0003-2442-5671 en_US http://dx.doi.org/10.1073/pnas.1108261109 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences of the United States of America PNAS |
| spellingShingle | Mower, Amanda F. Yu, Hongbo Majewska, Ania K. Okamoto, Ken-Ichi Hayashi, Yasunori Sur, Mriganka Kwok, Show Ming Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo |
| title | Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo |
| title_full | Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo |
| title_fullStr | Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo |
| title_full_unstemmed | Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo |
| title_short | Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo |
| title_sort | experience dependent regulation of camkii activity within single visual cortex synapses in vivo |
| url | http://hdl.handle.net/1721.1/73091 https://orcid.org/0000-0003-2442-5671 |
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