A microfabricated deformability-based flow cytometer with application to malaria
Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical re...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry
2012
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| Online Access: | http://hdl.handle.net/1721.1/73098 https://orcid.org/0000-0002-6223-6831 https://orcid.org/0000-0001-7215-1439 https://orcid.org/0000-0002-6250-8796 |
| _version_ | 1811090793199304704 |
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| author | Bow, Hansen Pivkin, Igor V. Diez Silva, Monica Goldfless, Stephen Jacob Dao, Ming Niles, Jacquin Suresh, Subra Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Bow, Hansen Pivkin, Igor V. Diez Silva, Monica Goldfless, Stephen Jacob Dao, Ming Niles, Jacquin Suresh, Subra Han, Jongyoon |
| author_sort | Bow, Hansen |
| collection | MIT |
| description | Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 10[superscript 3] to 10[superscript 4] individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner. |
| first_indexed | 2024-09-23T14:52:02Z |
| format | Article |
| id | mit-1721.1/73098 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:52:02Z |
| publishDate | 2012 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/730982022-10-01T23:01:02Z A microfabricated deformability-based flow cytometer with application to malaria Bow, Hansen Pivkin, Igor V. Diez Silva, Monica Goldfless, Stephen Jacob Dao, Ming Niles, Jacquin Suresh, Subra Han, Jongyoon Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Materials Science and Engineering Bow, Hansen Pivkin, Igor V. Diez Silva, Monica Goldfless, Stephen Jacob Dao, Ming Niles, Jacquin Suresh, Subra Han, Jongyoon Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 10[superscript 3] to 10[superscript 4] individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner. National Institutes of Health (U.S.) (Grant R01 HL094270-01A1) National Institutes of Health (U.S.) (Grant 1-R01-GM076689-01) Singapore-MIT Alliance for Research and Technology Center 2012-09-21T16:00:29Z 2012-09-21T16:00:29Z 2011-02 2010-10 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/73098 Bow, Hansen et al. “A Microfabricated Deformability-based Flow Cytometer with Application to Malaria.” Lab on a Chip 11.6 (2011): 1065. https://orcid.org/0000-0002-6223-6831 https://orcid.org/0000-0001-7215-1439 https://orcid.org/0000-0002-6250-8796 en_US http://dx.doi.org/10.1039/c0lc00472c Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Royal Society of Chemistry PubMed Central |
| spellingShingle | Bow, Hansen Pivkin, Igor V. Diez Silva, Monica Goldfless, Stephen Jacob Dao, Ming Niles, Jacquin Suresh, Subra Han, Jongyoon A microfabricated deformability-based flow cytometer with application to malaria |
| title | A microfabricated deformability-based flow cytometer with application to malaria |
| title_full | A microfabricated deformability-based flow cytometer with application to malaria |
| title_fullStr | A microfabricated deformability-based flow cytometer with application to malaria |
| title_full_unstemmed | A microfabricated deformability-based flow cytometer with application to malaria |
| title_short | A microfabricated deformability-based flow cytometer with application to malaria |
| title_sort | microfabricated deformability based flow cytometer with application to malaria |
| url | http://hdl.handle.net/1721.1/73098 https://orcid.org/0000-0002-6223-6831 https://orcid.org/0000-0001-7215-1439 https://orcid.org/0000-0002-6250-8796 |
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