Pan-Src Family Kinase Inhibitors Replace Sox2 during the Direct Reprogramming of Somatic Cells

Pan-Src Family Kinase Inhibitors Replace Sox2 during the Direct Reprogramming of Somatic Cells

Small molecules do the job: Somatic cells are reprogrammed into iPS cells upon ectopic expression of Oct4, Sox2, Klf4 and c-Myc. Application of a cell-based, high-throughput chemical screen led to the identification of Src family kinase (SFK) inhibitors as chemical replacements for retroviral Sox2 d...

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Bibliographic Details
Main Authors: Staerk, Judith, Lyssiotis, Costas A., Medeiro, Lea A., Bollong, Michael, Foreman, Ruth K., Zhu, Shoutian, Garcia, Michael, Gao, Qing, Bouchez, Laure C., Lairson, Luke L., Charette, Bradley D., Supekova, Lubica, Janes, Jeffrey, Brinker, Achim, Cho, Charles Y., Jaenisch, Rudolf, Schultz, Peter G.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Wiley Blackwell (John Wiley & Sons) 2012
Online Access:http://hdl.handle.net/1721.1/73479
Description
Summary:Small molecules do the job: Somatic cells are reprogrammed into iPS cells upon ectopic expression of Oct4, Sox2, Klf4 and c-Myc. Application of a cell-based, high-throughput chemical screen led to the identification of Src family kinase (SFK) inhibitors as chemical replacements for retroviral Sox2 delivery. These compounds are used to study the mechanisms underlying direct reprogramming and may ultimately help to bring iPS cell technology one step closer to clinical application.

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