Electrically addressable vesicles: Tools for dielectrophoresis metrology
Dielectrophoresis (DEP) has emerged as an important tool for the manipulation of bioparticles ranging from the submicron to the tens of microns in size. Here we show the use of phospholipid vesicle electroformation techniques to develop a new class of test particles with specifically engineered elec...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2012
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| Online Access: | http://hdl.handle.net/1721.1/73575 https://orcid.org/0000-0001-8898-2296 |
| _version_ | 1811071725383712768 |
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| author | Desai, Salil P. Vahey, Michael D. Voldman, Joel |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science Desai, Salil P. Vahey, Michael D. Voldman, Joel |
| author_sort | Desai, Salil P. |
| collection | MIT |
| description | Dielectrophoresis (DEP) has emerged as an important tool for the manipulation of bioparticles ranging from the submicron to the tens of microns in size. Here we show the use of phospholipid vesicle electroformation techniques to develop a new class of test particles with specifically engineered electrical propserties to enable identifiable dielectrophoretic responses in microfabricated systems. These electrically addressable vesicles (EAVs) enable the creation of electrically distinct populations of test particles for DEP. EAVs offer control of both their inner aqueous core and outer membrane properties; by encapsulating solutions of different electrolyte strength inside the vesicle and by incorporating functionalized phospholipids containing poly(ethylene glycol) (PEG) brushes attached to their hydrophilic headgroup in the vesicle membrane, we demonstrate control of the vesicles’ electrical polarizabilities. This combined with the ability to encode information about the properties of the vesicle in its fluorescence signature forms the first steps toward the development of EAV populations as metrology tools for any DEP-based microsystem. |
| first_indexed | 2024-09-23T08:55:28Z |
| format | Article |
| id | mit-1721.1/73575 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:55:28Z |
| publishDate | 2012 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/735752022-09-30T12:11:21Z Electrically addressable vesicles: Tools for dielectrophoresis metrology Desai, Salil P. Vahey, Michael D. Voldman, Joel Institute for Medical Engineering and Science Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Desai, Salil P. Vahey, Michael D. Voldman, Joel Dielectrophoresis (DEP) has emerged as an important tool for the manipulation of bioparticles ranging from the submicron to the tens of microns in size. Here we show the use of phospholipid vesicle electroformation techniques to develop a new class of test particles with specifically engineered electrical propserties to enable identifiable dielectrophoretic responses in microfabricated systems. These electrically addressable vesicles (EAVs) enable the creation of electrically distinct populations of test particles for DEP. EAVs offer control of both their inner aqueous core and outer membrane properties; by encapsulating solutions of different electrolyte strength inside the vesicle and by incorporating functionalized phospholipids containing poly(ethylene glycol) (PEG) brushes attached to their hydrophilic headgroup in the vesicle membrane, we demonstrate control of the vesicles’ electrical polarizabilities. This combined with the ability to encode information about the properties of the vesicle in its fluorescence signature forms the first steps toward the development of EAV populations as metrology tools for any DEP-based microsystem. National Institutes of Health (U.S.) (Grant RR199652) National Institutes of Health (U.S.) (Grant EB005753) Merck/CSBi (Fellowship) Solomon Buchsbaum AT&T Research Fund 2012-10-03T18:16:29Z 2012-10-03T18:16:29Z 2009-02 Article http://purl.org/eprint/type/JournalArticle 0743-7463 1520-5827 http://hdl.handle.net/1721.1/73575 Desai, Salil P., Michael D. Vahey, and Joel Voldman. “Electrically Addressable Vesicles: Tools for Dielectrophoresis Metrology.” Langmuir 25.6 (2009): 3867–3875. https://orcid.org/0000-0001-8898-2296 en_US http://dx.doi.org/10.1021/la803330c Langmuir Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Chemical Society (ACS) PubMed Central |
| spellingShingle | Desai, Salil P. Vahey, Michael D. Voldman, Joel Electrically addressable vesicles: Tools for dielectrophoresis metrology |
| title | Electrically addressable vesicles: Tools for dielectrophoresis metrology |
| title_full | Electrically addressable vesicles: Tools for dielectrophoresis metrology |
| title_fullStr | Electrically addressable vesicles: Tools for dielectrophoresis metrology |
| title_full_unstemmed | Electrically addressable vesicles: Tools for dielectrophoresis metrology |
| title_short | Electrically addressable vesicles: Tools for dielectrophoresis metrology |
| title_sort | electrically addressable vesicles tools for dielectrophoresis metrology |
| url | http://hdl.handle.net/1721.1/73575 https://orcid.org/0000-0001-8898-2296 |
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