Large-scale analysis of neurite growth dynamics on micropatterned substrates
2011 September 15
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Royal Society of Chemistry, The
2012
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| Online Access: | http://hdl.handle.net/1721.1/73606 https://orcid.org/0000-0001-6038-3603 |
| _version_ | 1826198373394284544 |
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| author | Wissner-Gross, Zachary D. Scott, Mark Andrew Ku, David L. Ramaswamy, Priya |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Wissner-Gross, Zachary D. Scott, Mark Andrew Ku, David L. Ramaswamy, Priya |
| author_sort | Wissner-Gross, Zachary D. |
| collection | MIT |
| description | 2011 September 15 |
| first_indexed | 2024-09-23T11:03:52Z |
| format | Article |
| id | mit-1721.1/73606 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:03:52Z |
| publishDate | 2012 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/736062022-09-27T16:52:18Z Large-scale analysis of neurite growth dynamics on micropatterned substrates Wissner-Gross, Zachary D. Scott, Mark Andrew Ku, David L. Ramaswamy, Priya Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Wissner-Gross, Zachary D. Scott, Mark Andrew Ku, David L. Ramaswamy, Priya 2011 September 15 During both development and regeneration of the nervous system, neurons display complex growth dynamics, and several neurites compete to become the neuron's single axon. Numerous mathematical and biophysical models have been proposed to explain this competition, which remain experimentally unverified. Large-scale, precise, and repeatable measurements of neurite dynamics have been difficult to perform, since neurons have varying numbers of neurites, which themselves have complex morphologies. To overcome these challenges using a minimal number of primary neurons, we generated repeatable neuronal morphologies on a large scale using laser-patterned micron-wide stripes of adhesive proteins on an otherwise highly non-adherent substrate. By analyzing thousands of quantitative time-lapse measurements of highly reproducible neurite growth dynamics, we show that total neurite growth accelerates until neurons polarize, that immature neurites compete even at very short lengths, and that neuronal polarity exhibits a distinct transition as neurites grow. Proposed neurite growth models agree only partially with our experimental observations. We further show that simple yet specific modifications can significantly improve these models, but still do not fully predict the complex neurite growth behavior. Our high-content analysis puts significant and nontrivial constraints on possible mechanistic models of neurite growth and specification. The methodology presented here could also be employed in large-scale chemical and target-based screens on a variety of complex and subtle phenotypes for therapeutic discoveries using minimal numbers of primary neurons. United States. Dept. of Defense. (National Defense Science and Engineering Graduate Fellowship) 2012-10-04T16:35:17Z 2012-10-04T16:35:17Z 2010-09 2010-06 Article http://purl.org/eprint/type/JournalArticle 1757-9694 http://hdl.handle.net/1721.1/73606 Wissner-Gross, Zachary D. et al. “Large-scale Analysis of Neurite Growth Dynamics on Micropatterned Substrates.” Integrative Biology 3.1 (2011): 65. https://orcid.org/0000-0001-6038-3603 en_US http://dx.doi.org/10.1039/c0ib00058b Integrative Biology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Royal Society of Chemistry, The PubMed Central |
| spellingShingle | Wissner-Gross, Zachary D. Scott, Mark Andrew Ku, David L. Ramaswamy, Priya Large-scale analysis of neurite growth dynamics on micropatterned substrates |
| title | Large-scale analysis of neurite growth dynamics on micropatterned substrates |
| title_full | Large-scale analysis of neurite growth dynamics on micropatterned substrates |
| title_fullStr | Large-scale analysis of neurite growth dynamics on micropatterned substrates |
| title_full_unstemmed | Large-scale analysis of neurite growth dynamics on micropatterned substrates |
| title_short | Large-scale analysis of neurite growth dynamics on micropatterned substrates |
| title_sort | large scale analysis of neurite growth dynamics on micropatterned substrates |
| url | http://hdl.handle.net/1721.1/73606 https://orcid.org/0000-0001-6038-3603 |
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