Co-directional replication-transcription conflicts lead to replication restart
August 24, 2011
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/73612 https://orcid.org/0000-0002-8235-7227 |
| _version_ | 1826216517512986624 |
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| author | Merrikh, Houra Machon, Cristina Grainger, William H. Soultanas, Panos Grossman, Alan Davis |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Merrikh, Houra Machon, Cristina Grainger, William H. Soultanas, Panos Grossman, Alan Davis |
| author_sort | Merrikh, Houra |
| collection | MIT |
| description | August 24, 2011 |
| first_indexed | 2024-09-23T16:48:55Z |
| format | Article |
| id | mit-1721.1/73612 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:48:55Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/736122022-09-29T21:40:13Z Co-directional replication-transcription conflicts lead to replication restart Merrikh, Houra Machon, Cristina Grainger, William H. Soultanas, Panos Grossman, Alan Davis Massachusetts Institute of Technology. Department of Biology Merrikh, Houra Grossman, Alan D. August 24, 2011 Head-on encounters between the replication and transcription machineries on the lagging DNA strand can lead to replication fork arrest and genomic instability1, 2. To avoid head-on encounters, most genes, especially essential and highly transcribed genes, are encoded on the leading strand such that transcription and replication are co-directional. Virtually all bacteria have the highly expressed ribosomal RNA genes co-directional with replication3. In bacteria, co-directional encounters seem inevitable because the rate of replication is about 10–20-fold greater than the rate of transcription. However, these encounters are generally thought to be benign2, 4, 5, 6, 7, 8, 9. Biochemical analyses indicate that head-on encounters10 are more deleterious than co-directional encounters8 and that in both situations, replication resumes without the need for any auxiliary restart proteins, at least in vitro. Here we show that in vivo, co-directional transcription can disrupt replication, leading to the involvement of replication restart proteins. We found that highly transcribed rRNA genes are hotspots for co-directional conflicts between replication and transcription in rapidly growing Bacillus subtilis cells. We observed a transcription-dependent increase in association of the replicative helicase and replication restart proteins where head-on and co-directional conflicts occur. Our results indicate that there are co-directional conflicts between replication and transcription in vivo. Furthermore, in contrast to the findings in vitro, the replication restart machinery is involved in vivo in resolving potentially deleterious encounters due to head-on and co-directional conflicts. These conflicts probably occur in many organisms and at many chromosomal locations and help to explain the presence of important auxiliary proteins involved in replication restart and in helping to clear a path along the DNA for the replisome. Biotechnology and Biological Sciences Research Council (Great Britain) (Grant BB/E006450/1) Wellcome Trust (London, England) (Grant 091968/Z/10/Z) National Institutes of Health (U.S.) (Grant GM41934) National Institutes of Health (U.S.) (Postdoctoral Fellowship GM093408) Biotechnology and Biological Sciences Research Council (Great Britain) (Sabbatical Visit) 2012-10-04T18:16:14Z 2012-10-04T18:16:14Z 2011-02 2010-02 Article http://purl.org/eprint/type/JournalArticle 0028-0836 1476-4687 http://hdl.handle.net/1721.1/73612 Merrikh, Houra et al. “Co-directional Replication–transcription Conflicts Lead to Replication Restart.” Nature 470.7335 (2011): 554–557. https://orcid.org/0000-0002-8235-7227 en_US http://dx.doi.org/10.1038/nature09758 Nature Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group PMC |
| spellingShingle | Merrikh, Houra Machon, Cristina Grainger, William H. Soultanas, Panos Grossman, Alan Davis Co-directional replication-transcription conflicts lead to replication restart |
| title | Co-directional replication-transcription conflicts lead to replication restart |
| title_full | Co-directional replication-transcription conflicts lead to replication restart |
| title_fullStr | Co-directional replication-transcription conflicts lead to replication restart |
| title_full_unstemmed | Co-directional replication-transcription conflicts lead to replication restart |
| title_short | Co-directional replication-transcription conflicts lead to replication restart |
| title_sort | co directional replication transcription conflicts lead to replication restart |
| url | http://hdl.handle.net/1721.1/73612 https://orcid.org/0000-0002-8235-7227 |
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