Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo
January 1, 2011
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Wiley Blackwell (Blackwell Publishing)
2012
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| Online Access: | http://hdl.handle.net/1721.1/73616 https://orcid.org/0000-0002-8235-7227 |
| _version_ | 1811089214946672640 |
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| author | Smits, Wiep Klaas Goranov, Alexi I. Grossman, Alan Davis |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Smits, Wiep Klaas Goranov, Alexi I. Grossman, Alan Davis |
| author_sort | Smits, Wiep Klaas |
| collection | MIT |
| description | January 1, 2011 |
| first_indexed | 2024-09-23T14:15:39Z |
| format | Article |
| id | mit-1721.1/73616 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:15:39Z |
| publishDate | 2012 |
| publisher | Wiley Blackwell (Blackwell Publishing) |
| record_format | dspace |
| spelling | mit-1721.1/736162022-09-28T19:33:06Z Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo Smits, Wiep Klaas Goranov, Alexi I. Grossman, Alan Davis Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Smits, Wiep Klaas Goranov, Alexi I. Grossman, Alan D. January 1, 2011 The essential proteins DnaB, DnaD and DnaI of Bacillus subtilis are required for initiation, but not elongation, of DNA replication, and for replication restart at stalled forks. The interactions and functions of these proteins have largely been determined in vitro based on their roles in replication restart. During replication initiation in vivo, it is not known if these proteins, and the replication initiator DnaA, associate with oriC independently of each other by virtue of their DNA binding activities, as a (sub)complex like other loader proteins, or in a particular dependent order. We used temperature-sensitive mutants or a conditional degradation system to inactivate each protein and test for association of the other proteins with oriC in vivo. We found that there was a clear order of stable association with oriC; DnaA, DnaD, DnaB, and finally DnaI-mediated loading of helicase. The loading of helicase via stable intermediates resembles that of eukaryotes and the established hierarchy provides several potential regulatory points. The general approach described here can be used to analyse assembly of other complexes. Netherlands Organization for Scientific Research (Rubicon fellowship) National Institutes of Health (U.S.) (Public Health Service Grant GM41934) 2012-10-04T18:50:54Z 2012-10-04T18:50:54Z 2009-12 Article http://purl.org/eprint/type/JournalArticle 0950-382X 1365-2958 http://hdl.handle.net/1721.1/73616 Smits, Wiep Klaas, Alexi I. Goranov, and Alan D. Grossman. “Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.” Molecular Microbiology 75.2 (2010): 452–461. https://orcid.org/0000-0002-8235-7227 en_US http://dx.doi.org/10.1111/j.1365-2958.2009.06999.x Molecular Microbiology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Wiley Blackwell (Blackwell Publishing) PMC |
| spellingShingle | Smits, Wiep Klaas Goranov, Alexi I. Grossman, Alan Davis Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo |
| title | Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo |
| title_full | Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo |
| title_fullStr | Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo |
| title_full_unstemmed | Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo |
| title_short | Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo |
| title_sort | ordered association of helicase loader proteins with the bacillus subtilis origin of replication in vivo |
| url | http://hdl.handle.net/1721.1/73616 https://orcid.org/0000-0002-8235-7227 |
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