Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters
2010 September 13
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell (John Wiley & Sons)
2012
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| Online Access: | http://hdl.handle.net/1721.1/73713 https://orcid.org/0000-0002-5749-7869 https://orcid.org/0000-0002-8121-9519 |
| _version_ | 1826205307033878528 |
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| author | Lukovic, Elvedin Taylor, Elizabeth Vogel Imperiali, Barbara |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Lukovic, Elvedin Taylor, Elizabeth Vogel Imperiali, Barbara |
| author_sort | Lukovic, Elvedin |
| collection | MIT |
| description | 2010 September 13 |
| first_indexed | 2024-09-23T13:10:38Z |
| format | Article |
| id | mit-1721.1/73713 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:10:38Z |
| publishDate | 2012 |
| publisher | Wiley Blackwell (John Wiley & Sons) |
| record_format | dspace |
| spelling | mit-1721.1/737132022-10-01T13:32:09Z Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters Lukovic, Elvedin Taylor, Elizabeth Vogel Imperiali, Barbara Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Lukovic, Elvedin Taylor, Elizabeth Vogel Imperiali, Barbara 2010 September 13 The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification. National Institutes of Health (U.S.). Cell Migration Consortium (GM064346) National Science Foundation (U.S.). Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems (NSF-0070319) 2012-10-10T14:57:05Z 2012-10-10T14:57:05Z 2009-08 2009-06 Article http://purl.org/eprint/type/JournalArticle 1433-7851 1521-3773 http://hdl.handle.net/1721.1/73713 Luković, Elvedin, Elizabeth Vogel Taylor, and Barbara Imperiali. “Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters.” Angewandte Chemie International Edition 48.37 (2009): 6828–6831. https://orcid.org/0000-0002-5749-7869 https://orcid.org/0000-0002-8121-9519 en_US http://dx.doi.org/10.1002/anie.200902374 Angewandte Chemie International Edition Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Wiley Blackwell (John Wiley & Sons) PMC |
| spellingShingle | Lukovic, Elvedin Taylor, Elizabeth Vogel Imperiali, Barbara Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters |
| title | Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters |
| title_full | Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters |
| title_fullStr | Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters |
| title_full_unstemmed | Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters |
| title_short | Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters |
| title_sort | monitoring protein kinases in cellular media with highly selective chimeric reporters |
| url | http://hdl.handle.net/1721.1/73713 https://orcid.org/0000-0002-5749-7869 https://orcid.org/0000-0002-8121-9519 |
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