Metastable Pluripotent States in NOD Mouse Derived ES Cells
Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of blastocysts, whereas epiblast stem cells (EpiSCs) are derived from the post-implantation epiblast and display a restricted developmental potential. Here we characterize pluripotent states in the non-obese diabetic (NOD) mouse...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2012
|
| Online Access: | http://hdl.handle.net/1721.1/73889 |
| _version_ | 1826203529159639040 |
|---|---|
| author | Hanna, Jacob Markoulaki, Styliani Mitalipova, Maisam Cassady, John P. Staerk, Judith Carey, Bryce W. Lengner, Christopher J. Foreman, Ruth K. Love, Jennifer Gao, Qing Kim, Jongpil Jaenisch, Rudolf Cheng, Albert Wu |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Hanna, Jacob Markoulaki, Styliani Mitalipova, Maisam Cassady, John P. Staerk, Judith Carey, Bryce W. Lengner, Christopher J. Foreman, Ruth K. Love, Jennifer Gao, Qing Kim, Jongpil Jaenisch, Rudolf Cheng, Albert Wu |
| author_sort | Hanna, Jacob |
| collection | MIT |
| description | Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of blastocysts, whereas
epiblast stem cells (EpiSCs) are derived from the post-implantation epiblast and display a restricted developmental potential. Here we characterize pluripotent states in the non-obese diabetic (NOD) mouse strain, which prior to this study was considered “non-permissive” for ES cell derivation. We find that NOD stem cells can be stabilized by providing constitutive expression of Klf4 or c-Myc or small molecules that can replace these factors during in vitro reprogramming. The NOD ES and iPS cells appear “metastable”, as they acquire an alternative EpiSC-like identity after removal of the exogenous factors, while their reintroduction converts the cells back to ICM-like pluripotency. Our findings suggest that stem cells from different genetic backgrounds can assume distinct states of pluripotency in vitro, the stability of which is regulated by endogenous genetic determinants and can be modified by exogenous factors. |
| first_indexed | 2024-09-23T12:38:28Z |
| format | Article |
| id | mit-1721.1/73889 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:38:28Z |
| publishDate | 2012 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/738892022-10-01T10:13:16Z Metastable Pluripotent States in NOD Mouse Derived ES Cells Hanna, Jacob Markoulaki, Styliani Mitalipova, Maisam Cassady, John P. Staerk, Judith Carey, Bryce W. Lengner, Christopher J. Foreman, Ruth K. Love, Jennifer Gao, Qing Kim, Jongpil Jaenisch, Rudolf Cheng, Albert Wu Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biology Cheng, Albert W. Cassady, John P. Foreman, Ruth K. Jaenisch, Rudolf Carey, Bryce W. Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of blastocysts, whereas epiblast stem cells (EpiSCs) are derived from the post-implantation epiblast and display a restricted developmental potential. Here we characterize pluripotent states in the non-obese diabetic (NOD) mouse strain, which prior to this study was considered “non-permissive” for ES cell derivation. We find that NOD stem cells can be stabilized by providing constitutive expression of Klf4 or c-Myc or small molecules that can replace these factors during in vitro reprogramming. The NOD ES and iPS cells appear “metastable”, as they acquire an alternative EpiSC-like identity after removal of the exogenous factors, while their reintroduction converts the cells back to ICM-like pluripotency. Our findings suggest that stem cells from different genetic backgrounds can assume distinct states of pluripotency in vitro, the stability of which is regulated by endogenous genetic determinants and can be modified by exogenous factors. National Institutes of Health (U.S.) (Grant RO1-HDO45022) National Institutes of Health (U.S.) (Grant R37-CA084198) National Institutes of Health (U.S.) (Grant RO1-CA087869) 2012-10-11T18:46:53Z 2012-10-11T18:46:53Z 2009-05 2009-04 Article http://purl.org/eprint/type/JournalArticle 1934-5909 http://hdl.handle.net/1721.1/73889 Hanna, Jacob et al. “Metastable Pluripotent States in NOD-Mouse-Derived ESCs.” Cell Stem Cell 4.6 (2009): 513–524. en_US http://dx.doi.org/10.1016/j.stem.2009.04.015 Cell Stem Cell Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Elsevier PMC |
| spellingShingle | Hanna, Jacob Markoulaki, Styliani Mitalipova, Maisam Cassady, John P. Staerk, Judith Carey, Bryce W. Lengner, Christopher J. Foreman, Ruth K. Love, Jennifer Gao, Qing Kim, Jongpil Jaenisch, Rudolf Cheng, Albert Wu Metastable Pluripotent States in NOD Mouse Derived ES Cells |
| title | Metastable Pluripotent States in NOD Mouse Derived ES Cells |
| title_full | Metastable Pluripotent States in NOD Mouse Derived ES Cells |
| title_fullStr | Metastable Pluripotent States in NOD Mouse Derived ES Cells |
| title_full_unstemmed | Metastable Pluripotent States in NOD Mouse Derived ES Cells |
| title_short | Metastable Pluripotent States in NOD Mouse Derived ES Cells |
| title_sort | metastable pluripotent states in nod mouse derived es cells |
| url | http://hdl.handle.net/1721.1/73889 |
| work_keys_str_mv | AT hannajacob metastablepluripotentstatesinnodmousederivedescells AT markoulakistyliani metastablepluripotentstatesinnodmousederivedescells AT mitalipovamaisam metastablepluripotentstatesinnodmousederivedescells AT cassadyjohnp metastablepluripotentstatesinnodmousederivedescells AT staerkjudith metastablepluripotentstatesinnodmousederivedescells AT careybrycew metastablepluripotentstatesinnodmousederivedescells AT lengnerchristopherj metastablepluripotentstatesinnodmousederivedescells AT foremanruthk metastablepluripotentstatesinnodmousederivedescells AT lovejennifer metastablepluripotentstatesinnodmousederivedescells AT gaoqing metastablepluripotentstatesinnodmousederivedescells AT kimjongpil metastablepluripotentstatesinnodmousederivedescells AT jaenischrudolf metastablepluripotentstatesinnodmousederivedescells AT chengalbertwu metastablepluripotentstatesinnodmousederivedescells |