Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units
BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note that we use the term “promoter” here to refer to both the promoter and the 5’...
| Main Authors: | , , , , , , , , , |
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| Format: | Technical Report |
| Language: | en_US |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/73912 |
| _version_ | 1811082164425457664 |
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| author | Boeke, Jef Mitchell, Leslie Cai, Yizhi Stracquadanio, Giovanni Chuang, James Tan, Scott Heston, Margo Wang, Jiarui Kim, Dong Won Noronha, Anne Marie |
| author_facet | Boeke, Jef Mitchell, Leslie Cai, Yizhi Stracquadanio, Giovanni Chuang, James Tan, Scott Heston, Margo Wang, Jiarui Kim, Dong Won Noronha, Anne Marie |
| author_sort | Boeke, Jef |
| collection | MIT |
| description | BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note that we use the term “promoter” here to refer to both the promoter and the 5’ UTR, which we currently define as a single part. Future iterations of this standard will incorporate subdivision of currently defined parts e.g. into promoter and 5’ UTR. The standard makes use of the type IIS restriction enzyme BsaI to generate standardized and user‐defined ‘signature overhangs’, thus enabling directional and seamless TU assembly. RFC88 is supported by the Yeast Standardized Collection of Parts for Expression (SCoPE), a repository of subcloned and sequence verified parts compatible with this assembly standard. The Yeast SCoPE is currently populated by a large number of S. cerevisiae promoters and terminators that facilitate expression and characterization of non‐native ORFs. |
| first_indexed | 2024-09-23T11:58:35Z |
| format | Technical Report |
| id | mit-1721.1/73912 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:58:35Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | mit-1721.1/739122019-04-12T21:12:08Z Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units Boeke, Jef Mitchell, Leslie Cai, Yizhi Stracquadanio, Giovanni Chuang, James Tan, Scott Heston, Margo Wang, Jiarui Kim, Dong Won Noronha, Anne Marie DNA assembly yeast BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note that we use the term “promoter” here to refer to both the promoter and the 5’ UTR, which we currently define as a single part. Future iterations of this standard will incorporate subdivision of currently defined parts e.g. into promoter and 5’ UTR. The standard makes use of the type IIS restriction enzyme BsaI to generate standardized and user‐defined ‘signature overhangs’, thus enabling directional and seamless TU assembly. RFC88 is supported by the Yeast Standardized Collection of Parts for Expression (SCoPE), a repository of subcloned and sequence verified parts compatible with this assembly standard. The Yeast SCoPE is currently populated by a large number of S. cerevisiae promoters and terminators that facilitate expression and characterization of non‐native ORFs. 2012-10-12T02:40:06Z 2012-10-12T02:40:06Z 2012-10-11 Technical Report http://hdl.handle.net/1721.1/73912 en_US BBF RFC;88 Attribution-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nd/3.0/us/ application/pdf |
| spellingShingle | DNA assembly yeast Boeke, Jef Mitchell, Leslie Cai, Yizhi Stracquadanio, Giovanni Chuang, James Tan, Scott Heston, Margo Wang, Jiarui Kim, Dong Won Noronha, Anne Marie Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units |
| title | Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units |
| title_full | Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units |
| title_fullStr | Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units |
| title_full_unstemmed | Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units |
| title_short | Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units |
| title_sort | yeast golden gate standardized assembly of s cerevisiae transcriptional units |
| topic | DNA assembly yeast |
| url | http://hdl.handle.net/1721.1/73912 |
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