Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units

Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units

BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note that we use the term “promoter” here to refer to both the promoter and the 5’...

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Bibliographic Details
Main Authors: Boeke, Jef, Mitchell, Leslie, Cai, Yizhi, Stracquadanio, Giovanni, Chuang, James, Tan, Scott, Heston, Margo, Wang, Jiarui, Kim, Dong Won, Noronha, Anne Marie
Format: Technical Report
Language:en_US
Published: 2012
Subjects:
DNA assembly
yeast
Online Access:http://hdl.handle.net/1721.1/73912

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