Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour
available in PMC 2012 June 22.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/73951 https://orcid.org/0000-0002-4109-5152 |
| _version_ | 1811071879289503744 |
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| author | Chang, Howard C. Paek, Jennifer Kim, Dennis H. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Chang, Howard C. Paek, Jennifer Kim, Dennis H. |
| author_sort | Chang, Howard C. |
| collection | MIT |
| description | available in PMC 2012 June 22. |
| first_indexed | 2024-09-23T08:57:24Z |
| format | Article |
| id | mit-1721.1/73951 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:57:24Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/739512021-09-09T17:02:24Z Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour Chang, Howard C. Paek, Jennifer Kim, Dennis H. Massachusetts Institute of Technology. Department of Biology Chang, Howard C. Paek, Jennifer Kim, Dennis H. available in PMC 2012 June 22. Heritable variation in behavioural traits generally has a complex genetic basis1, and thus naturally occurring polymorphisms that influence behaviour have been defined in only rare instances2,3. The isolation of wild strains of Caenorhabditis elegans has facilitated the study of natural genetic variation in this species4 and provided insights into its diverse microbial ecology5. C. elegans responds to bacterial infection with conserved innate immune responses6-8 and, while lacking the immunological memory of vertebrate adaptive immunity, exhibits an aversive learning response to pathogenic bacteria9. Here, we report the molecular characterization of naturally occurring coding polymorphisms in a C. elegans gene encoding a conserved HECT domain-containing E3 ubiquitin ligase, HECW-1. We show that two distinct polymorphisms in neighbouring residues of HECW-1 each affect C. elegans behavioural avoidance of a lawn of Pseudomonas aeruginosa. Neuronspecific rescue and ablation experiments, and genetic interaction analysis suggest that HECW-1 functions in a pair of sensory neurons to inhibit P. aeruginosa lawn avoidance behaviour through inhibition of the neuropeptide receptor NPR-110, which we have previously shown promotes P. aeruginosa lawn avoidance behaviour11. Our data establish a molecular basis for natural variation in a C. elegans behaviour that may undergo adaptive changes in response to microbial pathogens. National Institutes of Health (U.S.) (NIH Grant GM084477) 2012-10-15T13:59:54Z 2012-10-15T13:59:54Z 2011-12 Article http://purl.org/eprint/type/JournalArticle 0028-0836 1476-4687 http://hdl.handle.net/1721.1/73951 Chang, Howard C., Jennifer Paek, and Dennis H. Kim. “Natural Polymorphisms in C. Elegans HECW-1 E3 Ligase Affect Pathogen Avoidance Behaviour.” Nature 480.737 (2011): 525–529. https://orcid.org/0000-0002-4109-5152 en_US http://dx.doi.org/10.1038/nature10643 Nature Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Chang, Howard C. Paek, Jennifer Kim, Dennis H. Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour |
| title | Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour |
| title_full | Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour |
| title_fullStr | Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour |
| title_full_unstemmed | Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour |
| title_short | Natural polymorphisms in C. elegans HECW-1 E3 ligase affect pathogen avoidance behaviour |
| title_sort | natural polymorphisms in c elegans hecw 1 e3 ligase affect pathogen avoidance behaviour |
| url | http://hdl.handle.net/1721.1/73951 https://orcid.org/0000-0002-4109-5152 |
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