Laser-evoked synaptic transmission in cultured hippocampal neurons channelrhodopsin-2 delivered by adeno-associated virus
We present a method for studying synaptic transmission in mass cultures of dissociated hippocampal neurons based on patch clamp recording combined with laser stimulation of neurons expressing channelrhodopsin-2 (ChR2). Our goal was to use the high spatial resolution of laser illumination to come as...
Main Authors: | , , |
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Other Authors: | |
Format: | Article |
Language: | en_US |
Published: |
Elsevier
2012
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Online Access: | http://hdl.handle.net/1721.1/74024 |