Laser-evoked synaptic transmission in cultured hippocampal neurons channelrhodopsin-2 delivered by adeno-associated virus

We present a method for studying synaptic transmission in mass cultures of dissociated hippocampal neurons based on patch clamp recording combined with laser stimulation of neurons expressing channelrhodopsin-2 (ChR2). Our goal was to use the high spatial resolution of laser illumination to come as...

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Bibliographic Details
Main Authors: Wang, Jennifer, Hasan, Mazahir T., Seung, H. Sebastian
Other Authors: Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Format: Article
Language:en_US
Published: Elsevier 2012
Online Access:http://hdl.handle.net/1721.1/74024

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