Detecting and Understanding the Roles of Nitric Oxide in biology
We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2012
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| Online Access: | http://hdl.handle.net/1721.1/74072 https://orcid.org/0000-0002-2693-4982 |
| _version_ | 1826200847662448640 |
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| author | Tonzetich, Zachary J. McQuade, Lindsey E. Lippard, Stephen J. |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Tonzetich, Zachary J. McQuade, Lindsey E. Lippard, Stephen J. |
| author_sort | Tonzetich, Zachary J. |
| collection | MIT |
| description | We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron−sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron−sulfur proteins. Our studies reveal that NO disassembles the Fe−S clusters to form dinitrosyl iron complexes. |
| first_indexed | 2024-09-23T11:42:41Z |
| format | Article |
| id | mit-1721.1/74072 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:42:41Z |
| publishDate | 2012 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/740722022-10-01T05:24:43Z Detecting and Understanding the Roles of Nitric Oxide in biology Tonzetich, Zachary J. McQuade, Lindsey E. Lippard, Stephen J. Massachusetts Institute of Technology. Department of Chemistry Lippard, Stephen J. Tonzetich, Zachary J. McQuade, Lindsey E. Lippard, Stephen J. We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron−sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron−sulfur proteins. Our studies reveal that NO disassembles the Fe−S clusters to form dinitrosyl iron complexes. National Science Foundation (U.S.) (CHE-0907905) National Institute of General Medical Sciences (U.S.) (Grant F32 GM082031-03) 2012-10-18T14:45:27Z 2012-10-18T14:45:27Z 2010-07 Article http://purl.org/eprint/type/JournalArticle 0020-1669 1520-510X http://hdl.handle.net/1721.1/74072 Tonzetich, Zachary J., Lindsey E. McQuade, and Stephen J. Lippard. “Detecting and Understanding the Roles of Nitric Oxide in Biology.” Inorganic Chemistry 49.14 (2010): 6338–6348. https://orcid.org/0000-0002-2693-4982 en_US http://dx.doi.org/10.1021/ic9022757 Inorganic Chemistry Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Chemical Society (ACS) Prof. Lippard via Erja Kajosalo |
| spellingShingle | Tonzetich, Zachary J. McQuade, Lindsey E. Lippard, Stephen J. Detecting and Understanding the Roles of Nitric Oxide in biology |
| title | Detecting and Understanding the Roles of Nitric Oxide in biology |
| title_full | Detecting and Understanding the Roles of Nitric Oxide in biology |
| title_fullStr | Detecting and Understanding the Roles of Nitric Oxide in biology |
| title_full_unstemmed | Detecting and Understanding the Roles of Nitric Oxide in biology |
| title_short | Detecting and Understanding the Roles of Nitric Oxide in biology |
| title_sort | detecting and understanding the roles of nitric oxide in biology |
| url | http://hdl.handle.net/1721.1/74072 https://orcid.org/0000-0002-2693-4982 |
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