Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells
July 15, 2010
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Association of Immunologists
2012
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| Online Access: | http://hdl.handle.net/1721.1/74262 https://orcid.org/0000-0002-1090-6071 |
| _version_ | 1826197315105325056 |
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| author | Ploegh, Hidde Costantino, Cristina Maria Hafler, David A. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Ploegh, Hidde Costantino, Cristina Maria Hafler, David A. |
| author_sort | Ploegh, Hidde |
| collection | MIT |
| description | July 15, 2010 |
| first_indexed | 2024-09-23T10:45:50Z |
| format | Article |
| id | mit-1721.1/74262 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:45:50Z |
| publishDate | 2012 |
| publisher | American Association of Immunologists |
| record_format | dspace |
| spelling | mit-1721.1/742622022-09-30T22:50:10Z Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells Ploegh, Hidde Costantino, Cristina Maria Hafler, David A. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Ploegh, Hidde July 15, 2010 Although it has long been known that human CD4+ T cells can express functional class II MHC molecules, the role of lysosomal proteases in the T cell class II MHC processing and presentation pathway is unknown. Using CD4+ T cell clones that constitutively express class II MHC, we determined that cathepsin S is necessary for invariant chain proteolysis in T cells. CD4+HLA-DR+ T cells down-regulated cathepsin S expression and activity 18 h after activation, thereby ceasing nascent class II MHC product formation. This blockade resulted in the loss of the invariant chain fragment CLIP from the cell surface, suggesting that—like professional APC—CD4+ HLA-DR+ cells modulate self-Ag presentation as a consequence of activation. Furthermore, cathepsin S expression and activity, and concordantly cell surface CLIP expression, was reduced in HLA-DR+ CD4+ T cells as compared with B cells both in vitro and ex vivo. 2012-10-25T20:12:30Z 2012-10-25T20:12:30Z 2009-06 2009-03 Article http://purl.org/eprint/type/JournalArticle 0022-1767 1550-6606 http://hdl.handle.net/1721.1/74262 Costantino, C. M., H. L. Ploegh, and D. A. Hafler. “Cathepsin S Regulates Class II MHC Processing in Human CD4+ HLA-DR+ T Cells.” The Journal of Immunology 183.2 (2009): 945–952. https://orcid.org/0000-0002-1090-6071 en_US http://dx.doi.org/10.4049/jimmunol.0900921 Journal of Immunology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Association of Immunologists PMC |
| spellingShingle | Ploegh, Hidde Costantino, Cristina Maria Hafler, David A. Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells |
| title | Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells |
| title_full | Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells |
| title_fullStr | Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells |
| title_full_unstemmed | Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells |
| title_short | Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells |
| title_sort | cathepsin s regulates class ii mhc processing in human cd4 hla dr t cells |
| url | http://hdl.handle.net/1721.1/74262 https://orcid.org/0000-0002-1090-6071 |
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