Global gene disruption in human cells to assign genes to phenotypes

Global gene disruption in human cells to assign genes to phenotypes

Insertional mutagenesis in a haploid background can disrupt gene function[superscript 1]. We extend our earlier work by using a retroviral gene-trap vector to generate insertions in >98% of the genes expressed in a human cancer cell line that is haploid for all but one of its chromosomes. We appl...

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Bibliographic Details
Main Authors: Ploegh, Hidde, Yuan, Bingbing B., Carette, Jan E., Guimaraes, Carla P., Wuethrich, Irene, Blomen, Vincent A., Varadarajan, Malini, Sun, Chong, Bell, George W., Muellner, Markus K., Nijman, Sebastian M., Brummelkamp, Thijn R.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Nature Publishing Group 2012
Online Access:http://hdl.handle.net/1721.1/74500
https://orcid.org/0000-0002-1090-6071

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