LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion
Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/74512 https://orcid.org/0000-0002-3829-5612 |
| _version_ | 1811084049228234752 |
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| author | Tzur, Yonatan B. Carvalho, Carlos Egydio de Nadarajan, Saravanapriah Van Bostelen, Ivo Gu, Yanjie Chu, Diana S. Colaiacovo, Monica P. Cheeseman, Iain M |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Tzur, Yonatan B. Carvalho, Carlos Egydio de Nadarajan, Saravanapriah Van Bostelen, Ivo Gu, Yanjie Chu, Diana S. Colaiacovo, Monica P. Cheeseman, Iain M |
| author_sort | Tzur, Yonatan B. |
| collection | MIT |
| description | Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program. |
| first_indexed | 2024-09-23T12:43:51Z |
| format | Article |
| id | mit-1721.1/74512 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:43:51Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/745122022-09-28T09:41:02Z LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion Tzur, Yonatan B. Carvalho, Carlos Egydio de Nadarajan, Saravanapriah Van Bostelen, Ivo Gu, Yanjie Chu, Diana S. Colaiacovo, Monica P. Cheeseman, Iain M Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Cheeseman, Iain McPherson Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program. 2012-10-30T16:47:43Z 2012-10-30T16:47:43Z 2012-08 2012-01 Article http://purl.org/eprint/type/JournalArticle 1544-9173 1545-7885 http://hdl.handle.net/1721.1/74512 Tzur, Yonatan B. et al. “LAB-1 Targets PP1 and Restricts Aurora B Kinase Upon Entrance into Meiosis to Promote Sister Chromatid Cohesion.” Ed. R. Scott Hawley. PLoS Biology 10.8 (2012): e1001378. https://orcid.org/0000-0002-3829-5612 en_US http://dx.doi.org/10.1371/journal.pbio.1001378 PLoS Biology Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Tzur, Yonatan B. Carvalho, Carlos Egydio de Nadarajan, Saravanapriah Van Bostelen, Ivo Gu, Yanjie Chu, Diana S. Colaiacovo, Monica P. Cheeseman, Iain M LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion |
| title | LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion |
| title_full | LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion |
| title_fullStr | LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion |
| title_full_unstemmed | LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion |
| title_short | LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion |
| title_sort | lab 1 targets pp1 and restricts aurora b kinase upon entrance into meiosis to promote sister chromatid cohesion |
| url | http://hdl.handle.net/1721.1/74512 https://orcid.org/0000-0002-3829-5612 |
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