Dynamic single cell imaging of direct reprogramming reveals an early specifying event
available in PMC 2010 November 1.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/74519 https://orcid.org/0000-0001-8567-2049 |
| _version_ | 1811081038166753280 |
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| author | Smith, Zachary D. Nachman, Iftach Regev, Aviv Meissner, Alexander |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Smith, Zachary D. Nachman, Iftach Regev, Aviv Meissner, Alexander |
| author_sort | Smith, Zachary D. |
| collection | MIT |
| description | available in PMC 2010 November 1. |
| first_indexed | 2024-09-23T11:40:45Z |
| format | Article |
| id | mit-1721.1/74519 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:40:45Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/745192022-09-27T21:09:32Z Dynamic single cell imaging of direct reprogramming reveals an early specifying event Smith, Zachary D. Nachman, Iftach Regev, Aviv Meissner, Alexander Massachusetts Institute of Technology. Department of Biology Regev, Aviv available in PMC 2010 November 1. The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblasts (MEFs) to pluripotency factor–positive colonies. This enabled us to calculate a normalized cell-of-origin reprogramming efficiency that takes into account only the initial MEFs that respond to form reprogrammed colonies rather than the larger number of final colonies. Furthermore, this retrospective analysis revealed that successfully reprogramming cells undergo a rapid shift in their proliferative rate that coincides with a reduction in cellular area. This event occurs as early as the first cell division and with similar kinetics in all cells that form induced pluripotent stem (iPS) cell colonies. These data contribute to the theoretical modeling of reprogramming and suggest that certain parts of the reprogramming process follow defined rather than stochastic steps. Burroughs Wellcome Fund (Career Award at the Scientific Interface) Pew Charitable Trusts Massachusetts Life Sciences Center (New Investigator grant) Broad Institute (Investigator of the Merkin Foundation for Stem Cell Research) Howard Hughes Medical Institute (Early Career Scientist) Alfred P. Sloan Foundation National Institutes of Health (U.S.) (Pioneer Award) 2012-10-31T13:35:12Z 2012-10-31T13:35:12Z 2010-05 2010-03 Article http://purl.org/eprint/type/JournalArticle 1087-0156 1546-1696 http://hdl.handle.net/1721.1/74519 Smith, Zachary D et al. “Dynamic Single-cell Imaging of Direct Reprogramming Reveals an Early Specifying Event.” Nature Biotechnology 28.5 (2010): 521–526. Web. https://orcid.org/0000-0001-8567-2049 en_US http://dx.doi.org/10.1038/nbt.1632 Nature Biotechnology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group PMC |
| spellingShingle | Smith, Zachary D. Nachman, Iftach Regev, Aviv Meissner, Alexander Dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| title | Dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| title_full | Dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| title_fullStr | Dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| title_full_unstemmed | Dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| title_short | Dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| title_sort | dynamic single cell imaging of direct reprogramming reveals an early specifying event |
| url | http://hdl.handle.net/1721.1/74519 https://orcid.org/0000-0001-8567-2049 |
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