Chromatin signature of embryonic pluripotency is established during genome activation
available in PMC 2011 April 8.
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/74520 https://orcid.org/0000-0001-8567-2049 |
| _version_ | 1826203607873093632 |
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| author | Vastenhouw, Nadine L. Zhang, Yong Woods, Ian G. Imam, Farhad Regev, Aviv Liu, X. Shirley Rinn, John L. Schier, Alexander F. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Vastenhouw, Nadine L. Zhang, Yong Woods, Ian G. Imam, Farhad Regev, Aviv Liu, X. Shirley Rinn, John L. Schier, Alexander F. |
| author_sort | Vastenhouw, Nadine L. |
| collection | MIT |
| description | available in PMC 2011 April 8. |
| first_indexed | 2024-09-23T12:39:58Z |
| format | Article |
| id | mit-1721.1/74520 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:39:58Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/745202022-10-01T10:22:58Z Chromatin signature of embryonic pluripotency is established during genome activation Vastenhouw, Nadine L. Zhang, Yong Woods, Ian G. Imam, Farhad Regev, Aviv Liu, X. Shirley Rinn, John L. Schier, Alexander F. Massachusetts Institute of Technology. Department of Biology Regev, Aviv available in PMC 2011 April 8. After fertilization the embryonic genome is inactive until transcription is initiated during the maternal–zygotic transition. This transition coincides with the formation of pluripotent cells, which in mammals can be used to generate embryonic stem cells. To study the changes in chromatin structure that accompany pluripotency and genome activation, we mapped the genomic locations of histone H3 molecules bearing lysine trimethylation modifications before and after the maternal–zygotic transition in zebrafish. Histone H3 lysine 27 trimethylation (H3K27me3), which is repressive, and H3K4me3, which is activating, were not detected before the transition. After genome activation, more than 80% of genes were marked by H3K4me3, including many inactive developmental regulatory genes that were also marked by H3K27me3. Sequential chromatin immunoprecipitation demonstrated that the same promoter regions had both trimethylation marks. Such bivalent chromatin domains also exist in embryonic stem cells and are thought to poise genes for activation while keeping them repressed. Furthermore, we found many inactive genes that were uniquely marked by H3K4me3. Despite this activating modification, these monovalent genes were neither expressed nor stably bound by RNA polymerase II. Inspection of published data sets revealed similar monovalent domains in embryonic stem cells. Moreover, H3K4me3 marks could form in the absence of both sequence-specific transcriptional activators and stable association of RNA polymerase II, as indicated by the analysis of an inducible transgene. These results indicate that bivalent and monovalent domains might poise embryonic genes for activation and that the chromatin profile associated with pluripotency is established during the maternal–zygotic transition. National Institutes of Health (U.S.) (grant 1R01 HG004069) National Institutes of Health (U.S.) (grant 5R01 GM56211) Human Frontier Science Program (Strasbourg, France) (LT-00090/2007) European Molecular Biology Organization (fellowship) 2012-10-31T13:55:42Z 2012-10-31T13:55:42Z 2010-03 2009-08 Article http://purl.org/eprint/type/JournalArticle 0028-0836 1476-4687 http://hdl.handle.net/1721.1/74520 Vastenhouw, Nadine L. et al. “Chromatin Signature of Embryonic Pluripotency Is Established During Genome Activation.” Nature 464.7290 (2010): 922–926. Web. https://orcid.org/0000-0001-8567-2049 en_US http://dx.doi.org/10.1038/nature08866 Nature Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group PMC |
| spellingShingle | Vastenhouw, Nadine L. Zhang, Yong Woods, Ian G. Imam, Farhad Regev, Aviv Liu, X. Shirley Rinn, John L. Schier, Alexander F. Chromatin signature of embryonic pluripotency is established during genome activation |
| title | Chromatin signature of embryonic pluripotency is established during genome activation |
| title_full | Chromatin signature of embryonic pluripotency is established during genome activation |
| title_fullStr | Chromatin signature of embryonic pluripotency is established during genome activation |
| title_full_unstemmed | Chromatin signature of embryonic pluripotency is established during genome activation |
| title_short | Chromatin signature of embryonic pluripotency is established during genome activation |
| title_sort | chromatin signature of embryonic pluripotency is established during genome activation |
| url | http://hdl.handle.net/1721.1/74520 https://orcid.org/0000-0001-8567-2049 |
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