Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain
The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleocytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein trans...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2012
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| Online Access: | http://hdl.handle.net/1721.1/74575 https://orcid.org/0000-0001-8012-1512 |
| _version_ | 1811087115979587584 |
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| author | Partridge, James R. Schwartz, Thomas |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Partridge, James R. Schwartz, Thomas |
| author_sort | Partridge, James R. |
| collection | MIT |
| description | The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleocytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein transport. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nup153 at the nuclear face and Nup358 at the cytoplasmic face. Both nucleoporins contain a stretch of distinct, Ran-binding zinc finger domains. Here, we present six crystal structures of Nup153-zinc fingers in complex with Ran and a 1.48 Å crystal structure of RanGDP. Crystal engineering allowed us to obtain well diffracting crystals so that all ZnF–Ran complex structures are refined to high resolution. Each of the four zinc finger modules of Nup153 binds one Ran molecule in apparently non-allosteric fashion. The affinity is measurably higher for RanGDP than for RanGTP and varies modestly between the individual zinc fingers. By microcalorimetric and mutational analysis, we determined that one specific hydrogen bond accounts for most of the differences in the binding affinity of individual zinc fingers. Genomic analysis reveals that only in animals do NPCs contain Ran-binding zinc fingers. We speculate that these organisms evolved a mechanism to maintain a high local concentration of Ran at the vicinity of the NPC, using this zinc finger domain as a sink. |
| first_indexed | 2024-09-23T13:40:22Z |
| format | Article |
| id | mit-1721.1/74575 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:40:22Z |
| publishDate | 2012 |
| publisher | Elsevier |
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| spelling | mit-1721.1/745752022-09-28T15:22:26Z Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain Partridge, James R. Schwartz, Thomas Massachusetts Institute of Technology. Department of Biology Partridge, James R. Schwartz, Thomas The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleocytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein transport. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nup153 at the nuclear face and Nup358 at the cytoplasmic face. Both nucleoporins contain a stretch of distinct, Ran-binding zinc finger domains. Here, we present six crystal structures of Nup153-zinc fingers in complex with Ran and a 1.48 Å crystal structure of RanGDP. Crystal engineering allowed us to obtain well diffracting crystals so that all ZnF–Ran complex structures are refined to high resolution. Each of the four zinc finger modules of Nup153 binds one Ran molecule in apparently non-allosteric fashion. The affinity is measurably higher for RanGDP than for RanGTP and varies modestly between the individual zinc fingers. By microcalorimetric and mutational analysis, we determined that one specific hydrogen bond accounts for most of the differences in the binding affinity of individual zinc fingers. Genomic analysis reveals that only in animals do NPCs contain Ran-binding zinc fingers. We speculate that these organisms evolved a mechanism to maintain a high local concentration of Ran at the vicinity of the NPC, using this zinc finger domain as a sink. 2012-11-06T17:04:12Z 2012-11-06T17:04:12Z 2009-06 2009-06 Article http://purl.org/eprint/type/JournalArticle 0022-2836 1089-8638 http://hdl.handle.net/1721.1/74575 Partridge, James R., and Thomas U. Schwartz. “Crystallographic and Biochemical Analysis of the Ran-binding Zinc Finger Domain.” Journal of Molecular Biology 391.2 (2009): 375–389. https://orcid.org/0000-0001-8012-1512 en_US http://dx.doi.org/10.1016/j.jmb.2009.06.011 Journal of Molecular Biology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Elsevier PMC |
| spellingShingle | Partridge, James R. Schwartz, Thomas Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain |
| title | Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain |
| title_full | Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain |
| title_fullStr | Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain |
| title_full_unstemmed | Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain |
| title_short | Crystallographic and Biochemical Analysis of the Ran-Binding Zinc Finger Domain |
| title_sort | crystallographic and biochemical analysis of the ran binding zinc finger domain |
| url | http://hdl.handle.net/1721.1/74575 https://orcid.org/0000-0001-8012-1512 |
| work_keys_str_mv | AT partridgejamesr crystallographicandbiochemicalanalysisoftheranbindingzincfingerdomain AT schwartzthomas crystallographicandbiochemicalanalysisoftheranbindingzincfingerdomain |