The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing
RNA splicing is a major regulatory mechanism for controlling eukaryotic gene expression. By generating various splice isoforms from a single pre–mRNA, alternative splicing plays a key role in promoting the evolving complexity of metazoans. Numerous splicing factors have been identified. However, the...
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/74635 https://orcid.org/0000-0002-9964-9613 |
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| author | Ma, Long Gao, Xiaoyang Luo, Jintao Huang, Liange Teng, Yanling Horvitz, H. Robert |
| author2 | move to dc.description.sponsorship |
| author_facet | move to dc.description.sponsorship Ma, Long Gao, Xiaoyang Luo, Jintao Huang, Liange Teng, Yanling Horvitz, H. Robert |
| author_sort | Ma, Long |
| collection | MIT |
| description | RNA splicing is a major regulatory mechanism for controlling eukaryotic gene expression. By generating various splice isoforms from a single pre–mRNA, alternative splicing plays a key role in promoting the evolving complexity of metazoans. Numerous splicing factors have been identified. However, the in vivo functions of many splicing factors remain to be understood. In vivo studies are essential for understanding the molecular mechanisms of RNA splicing and the biology of numerous RNA splicing-related diseases. We previously isolated a Caenorhabditis elegans mutant defective in an essential gene from a genetic screen for suppressors of the rubberband Unc phenotype of unc-93(e1500) animals. This mutant contains missense mutations in two adjacent codons of the C. elegans microfibrillar-associated protein 1 gene mfap-1. mfap-1(n4564 n5214) suppresses the Unc phenotypes of different rubberband Unc mutants in a pattern similar to that of mutations in the splicing factor genes uaf-1 (the C. elegans U2AF large subunit gene) and sfa-1 (the C. elegans SF1/BBP gene). We used the endogenous gene tos-1 as a reporter for splicing and detected increased intron 1 retention and exon 3 skipping of tos-1 transcripts in mfap-1(n4564 n5214) animals. Using a yeast two-hybrid screen, we isolated splicing factors as potential MFAP-1 interactors. Our studies indicate that C. elegans mfap-1 encodes a splicing factor that can affect alternative splicing. |
| first_indexed | 2024-09-23T13:45:25Z |
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| id | mit-1721.1/74635 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:45:25Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
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| spelling | mit-1721.1/746352022-09-28T15:55:23Z The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing Ma, Long Gao, Xiaoyang Luo, Jintao Huang, Liange Teng, Yanling Horvitz, H. Robert move to dc.description.sponsorship Massachusetts Institute of Technology. Department of Biology Horvitz, H. Robert RNA splicing is a major regulatory mechanism for controlling eukaryotic gene expression. By generating various splice isoforms from a single pre–mRNA, alternative splicing plays a key role in promoting the evolving complexity of metazoans. Numerous splicing factors have been identified. However, the in vivo functions of many splicing factors remain to be understood. In vivo studies are essential for understanding the molecular mechanisms of RNA splicing and the biology of numerous RNA splicing-related diseases. We previously isolated a Caenorhabditis elegans mutant defective in an essential gene from a genetic screen for suppressors of the rubberband Unc phenotype of unc-93(e1500) animals. This mutant contains missense mutations in two adjacent codons of the C. elegans microfibrillar-associated protein 1 gene mfap-1. mfap-1(n4564 n5214) suppresses the Unc phenotypes of different rubberband Unc mutants in a pattern similar to that of mutations in the splicing factor genes uaf-1 (the C. elegans U2AF large subunit gene) and sfa-1 (the C. elegans SF1/BBP gene). We used the endogenous gene tos-1 as a reporter for splicing and detected increased intron 1 retention and exon 3 skipping of tos-1 transcripts in mfap-1(n4564 n5214) animals. Using a yeast two-hybrid screen, we isolated splicing factors as potential MFAP-1 interactors. Our studies indicate that C. elegans mfap-1 encodes a splicing factor that can affect alternative splicing. National Natural Science Foundation (China) (Grant 30971639) United States. National Institutes of Health (Grant GM24663) 2012-11-14T15:17:15Z 2012-11-14T15:17:15Z 2012-07 2012-03 Article http://purl.org/eprint/type/JournalArticle 1553-7390 1553-7404 http://hdl.handle.net/1721.1/74635 Ma, Long et al. “The Caenorhabditis Elegans Gene Mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing.” Ed. Andrew D. Chisholm. PLoS Genetics 8.7 (2012). https://orcid.org/0000-0002-9964-9613 en_US http://dx.doi.org/10.1371/journal.pgen.1002827 PLoS Genetics Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Ma, Long Gao, Xiaoyang Luo, Jintao Huang, Liange Teng, Yanling Horvitz, H. Robert The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing |
| title | The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing |
| title_full | The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing |
| title_fullStr | The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing |
| title_full_unstemmed | The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing |
| title_short | The Caenorhabditis elegans Gene mfap-1 Encodes a Nuclear Protein That Affects Alternative Splicing |
| title_sort | caenorhabditis elegans gene mfap 1 encodes a nuclear protein that affects alternative splicing |
| url | http://hdl.handle.net/1721.1/74635 https://orcid.org/0000-0002-9964-9613 |
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