Protein Microarray: "Theory" to "Real Practice"
Fueled by ever-growing genomic information and rapid developments of proteomics–the large scale analysis of proteins and mapping its functional role has become one of the most important disciplines for characterizing complex cell function. For building functional linkages between the biomolecules, a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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2004
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| Online Access: | http://hdl.handle.net/1721.1/7489 |
| _version_ | 1811097963394498560 |
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| author | Ng, Jin Kiat Ajikumar, Parayil Kumaran Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon |
| author_facet | Ng, Jin Kiat Ajikumar, Parayil Kumaran Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon |
| author_sort | Ng, Jin Kiat |
| collection | MIT |
| description | Fueled by ever-growing genomic information and rapid developments of proteomics–the large scale analysis of proteins and mapping its functional role has become one of the most important disciplines for characterizing complex cell function. For building functional linkages between the biomolecules, and for providing insight into the mechanisms of biological processes, last decade witnessed the exploration of combinatorial and chip technology for the detection of bimolecules in a high throughput and spatially addressable fashion. Among the various techniques developed, the protein chip technology has been rapid. Recently we demonstrated a new platform called “Spacially addressable protein array” (SAPA) to profile the ligand receptor interactions. To optimize the platform, the present study investigated various parameters such as the surface chemistry and role of additives for achieving high density and high-throughput detection with minimal nonspecific protein adsorption. In summary the present poster will address some of the critical challenges in protein micro array technology and the process of fine tuning to achieve the optimum system for solving real biological problems. |
| first_indexed | 2024-09-23T17:07:27Z |
| format | Article |
| id | mit-1721.1/7489 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:07:27Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | mit-1721.1/74892019-04-11T02:45:25Z Protein Microarray: "Theory" to "Real Practice" Ng, Jin Kiat Ajikumar, Parayil Kumaran Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon protein microarray proteomics spacially addressable protein array SAPA Fueled by ever-growing genomic information and rapid developments of proteomics–the large scale analysis of proteins and mapping its functional role has become one of the most important disciplines for characterizing complex cell function. For building functional linkages between the biomolecules, and for providing insight into the mechanisms of biological processes, last decade witnessed the exploration of combinatorial and chip technology for the detection of bimolecules in a high throughput and spatially addressable fashion. Among the various techniques developed, the protein chip technology has been rapid. Recently we demonstrated a new platform called “Spacially addressable protein array” (SAPA) to profile the ligand receptor interactions. To optimize the platform, the present study investigated various parameters such as the surface chemistry and role of additives for achieving high density and high-throughput detection with minimal nonspecific protein adsorption. In summary the present poster will address some of the critical challenges in protein micro array technology and the process of fine tuning to achieve the optimum system for solving real biological problems. Singapore-MIT Alliance (SMA) 2004-12-16T04:43:51Z 2004-12-16T04:43:51Z 2005-01 Article http://hdl.handle.net/1721.1/7489 en Molecular Engineering of Biological and Chemical Systems (MEBCS); 12959 bytes application/pdf application/pdf |
| spellingShingle | protein microarray proteomics spacially addressable protein array SAPA Ng, Jin Kiat Ajikumar, Parayil Kumaran Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon Protein Microarray: "Theory" to "Real Practice" |
| title | Protein Microarray: "Theory" to "Real Practice" |
| title_full | Protein Microarray: "Theory" to "Real Practice" |
| title_fullStr | Protein Microarray: "Theory" to "Real Practice" |
| title_full_unstemmed | Protein Microarray: "Theory" to "Real Practice" |
| title_short | Protein Microarray: "Theory" to "Real Practice" |
| title_sort | protein microarray theory to real practice |
| topic | protein microarray proteomics spacially addressable protein array SAPA |
| url | http://hdl.handle.net/1721.1/7489 |
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