Mediator and cohesin connect gene expression and chromatin architecture
Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but t...
| Principais autores: | , , , , , , , , , , , , |
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| Outros Autores: | |
| Formato: | Artigo |
| Idioma: | en_US |
| Publicado em: |
Nature Publishing Group
2012
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| Acesso em linha: | http://hdl.handle.net/1721.1/75293 https://orcid.org/0000-0001-8855-8647 |
| _version_ | 1826211752470118400 |
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| author | Kagey, Michael H. Newman, Jamie Jennifer Bilodeau, Steve Zhan, Ye Orlando, David A. van Berkum, Nynke L. Ebmeier, Christopher C. Goossens, Jesse Rahl, Peter B. Levine, Stuart S. Taatjes, Dylan J. Dekker, Job Young, Richard A. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Kagey, Michael H. Newman, Jamie Jennifer Bilodeau, Steve Zhan, Ye Orlando, David A. van Berkum, Nynke L. Ebmeier, Christopher C. Goossens, Jesse Rahl, Peter B. Levine, Stuart S. Taatjes, Dylan J. Dekker, Job Young, Richard A. |
| author_sort | Kagey, Michael H. |
| collection | MIT |
| description | Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but this process is not well understood. Here we report that mediator and cohesin physically and functionally connect the enhancers and core promoters of active genes in murine embryonic stem cells. Mediator, a transcriptional coactivator, forms a complex with cohesin, which can form rings that connect two DNA segments. The cohesin-loading factor Nipbl is associated with mediator–cohesin complexes, providing a means to load cohesin at promoters. DNA looping is observed between the enhancers and promoters occupied by mediator and cohesin. Mediator and cohesin co-occupy different promoters in different cells, thus generating cell-type-specific DNA loops linked to the gene expression program of each cell. |
| first_indexed | 2024-09-23T15:10:53Z |
| format | Article |
| id | mit-1721.1/75293 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:10:53Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/752932022-10-02T01:10:17Z Mediator and cohesin connect gene expression and chromatin architecture Kagey, Michael H. Newman, Jamie Jennifer Bilodeau, Steve Zhan, Ye Orlando, David A. van Berkum, Nynke L. Ebmeier, Christopher C. Goossens, Jesse Rahl, Peter B. Levine, Stuart S. Taatjes, Dylan J. Dekker, Job Young, Richard A. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Kagey, Michael H. Newman, Jamie Jennifer Bilodeau, Steve Orlando, David A. Rahl, Peter B. Levine, Stuart S. Young, Richard A. Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but this process is not well understood. Here we report that mediator and cohesin physically and functionally connect the enhancers and core promoters of active genes in murine embryonic stem cells. Mediator, a transcriptional coactivator, forms a complex with cohesin, which can form rings that connect two DNA segments. The cohesin-loading factor Nipbl is associated with mediator–cohesin complexes, providing a means to load cohesin at promoters. DNA looping is observed between the enhancers and promoters occupied by mediator and cohesin. Mediator and cohesin co-occupy different promoters in different cells, thus generating cell-type-specific DNA loops linked to the gene expression program of each cell. National Institutes of Health (U.S.) (Fellowship) Canadian Institutes of Health Research (Research Fellowship) National Institutes of Health (U.S.) (Grant R01 HG002668) 2012-12-07T17:22:06Z 2012-12-07T17:22:06Z 2010-08 2010-05 Article http://purl.org/eprint/type/JournalArticle 0028-0836 1476-4687 http://hdl.handle.net/1721.1/75293 Kagey, Michael H. et al. “Mediator and Cohesin Connect Gene Expression and Chromatin Architecture.” Nature 467.7314 (2010): 430–435. https://orcid.org/0000-0001-8855-8647 en_US http://dx.doi.org/10.1038/nature09380 Nature Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group PMC |
| spellingShingle | Kagey, Michael H. Newman, Jamie Jennifer Bilodeau, Steve Zhan, Ye Orlando, David A. van Berkum, Nynke L. Ebmeier, Christopher C. Goossens, Jesse Rahl, Peter B. Levine, Stuart S. Taatjes, Dylan J. Dekker, Job Young, Richard A. Mediator and cohesin connect gene expression and chromatin architecture |
| title | Mediator and cohesin connect gene expression and chromatin architecture |
| title_full | Mediator and cohesin connect gene expression and chromatin architecture |
| title_fullStr | Mediator and cohesin connect gene expression and chromatin architecture |
| title_full_unstemmed | Mediator and cohesin connect gene expression and chromatin architecture |
| title_short | Mediator and cohesin connect gene expression and chromatin architecture |
| title_sort | mediator and cohesin connect gene expression and chromatin architecture |
| url | http://hdl.handle.net/1721.1/75293 https://orcid.org/0000-0001-8855-8647 |
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