Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells
Arthur Manuscript date: 2010 October 9
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2012
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| Online Access: | http://hdl.handle.net/1721.1/75294 https://orcid.org/0000-0001-8855-8647 |
| _version_ | 1826211448605376512 |
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| author | Juan, Aster H. Kumar, Roshan M. Marx, Joseph G. Young, Richard A. Sartorelli, Vittorio |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Juan, Aster H. Kumar, Roshan M. Marx, Joseph G. Young, Richard A. Sartorelli, Vittorio |
| author_sort | Juan, Aster H. |
| collection | MIT |
| description | Arthur Manuscript date: 2010 October 9 |
| first_indexed | 2024-09-23T15:06:02Z |
| format | Article |
| id | mit-1721.1/75294 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:06:02Z |
| publishDate | 2012 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/752942022-09-29T12:41:14Z Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells Juan, Aster H. Kumar, Roshan M. Marx, Joseph G. Young, Richard A. Sartorelli, Vittorio Massachusetts Institute of Technology. Department of Biology Young, Richard A. Arthur Manuscript date: 2010 October 9 Polycomb group (PcG) proteins exert essential functions in the most disparate biological processes. The contribution of PcG proteins to cell commitment and differentiation relates to their ability to repress transcription of developmental regulators in embryonic stem (ES) cells and in committed cell lineages, including skeletal muscle cells (SMC). PcG proteins are preferentially removed from transcribed regions, but the underlying mechanisms remain unclear. Here, PcG proteins are found to occupy and repress transcription from an intronic region containing the microRNA miR-214 in undifferentiated SMC. Differentiation coincides with PcG disengagement, recruitment of the developmental regulators MyoD and myogenin, and activation of miR-214 transcription. Once transcribed, miR-214 negatively feeds back on PcG by targeting the Ezh2 3′UTR, the catalytic subunit of the PRC2 complex. miR-214-mediated Ezh2 protein reduction accelerates SMC differentiation and promotes unscheduled transcription of developmental regulators in ES cells. Thus, miR-214 and Ezh2 establish a regulatory loop controlling PcG-dependent gene expression during differentiation. National Institute of Arthritis and Musculoskeletal and Skin Diseases (U.S.) (Intramural Research Program) 2012-12-07T17:32:44Z 2012-12-07T17:32:44Z 2009-10 2008-12 Article http://purl.org/eprint/type/JournalArticle 1097-2765 http://hdl.handle.net/1721.1/75294 Juan, Aster H. et al. “Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells.” Molecular Cell 36.1 (2009): 61–74. https://orcid.org/0000-0001-8855-8647 en_US http://dx.doi.org/10.1016/j.molcel.2009.08.008 Molecular Cell Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Elsevier PMC |
| spellingShingle | Juan, Aster H. Kumar, Roshan M. Marx, Joseph G. Young, Richard A. Sartorelli, Vittorio Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells |
| title | Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells |
| title_full | Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells |
| title_fullStr | Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells |
| title_full_unstemmed | Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells |
| title_short | Mir-214-Dependent Regulation of the Polycomb Protein Ezh2 in Skeletal Muscle and Embryonic Stem Cells |
| title_sort | mir 214 dependent regulation of the polycomb protein ezh2 in skeletal muscle and embryonic stem cells |
| url | http://hdl.handle.net/1721.1/75294 https://orcid.org/0000-0001-8855-8647 |
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