Temperature dependence of ssrA-tag mediated protein degradation
Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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BioMed Central Ltd.
2012
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| Online Access: | http://hdl.handle.net/1721.1/75371 https://orcid.org/0000-0002-2031-8871 |
| _version_ | 1826205367354261504 |
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| author | Purcell, Oliver Grierson, Claire S Bernardo, Mario Savery, Nigel J |
| author2 | Massachusetts Institute of Technology. Research Laboratory of Electronics |
| author_facet | Massachusetts Institute of Technology. Research Laboratory of Electronics Purcell, Oliver Grierson, Claire S Bernardo, Mario Savery, Nigel J |
| author_sort | Purcell, Oliver |
| collection | MIT |
| description | Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be subjected to variable conditions, and be required to display precise, quantitative dynamics, which will be more susceptible to environmental changes than the general qualitative dynamics focussed on so far. |
| first_indexed | 2024-09-23T13:11:53Z |
| format | Article |
| id | mit-1721.1/75371 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:11:53Z |
| publishDate | 2012 |
| publisher | BioMed Central Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/753712022-09-28T12:32:54Z Temperature dependence of ssrA-tag mediated protein degradation Purcell, Oliver Grierson, Claire S Bernardo, Mario Savery, Nigel J Massachusetts Institute of Technology. Research Laboratory of Electronics Purcell, Oliver Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be subjected to variable conditions, and be required to display precise, quantitative dynamics, which will be more susceptible to environmental changes than the general qualitative dynamics focussed on so far. Bristol Centre for Complexity Sciences (EPSRC-GB Grant No. EP/E501214/1) 2012-12-11T15:41:24Z 2012-12-11T15:41:24Z 2012-07 2012-02 2012-12-01T16:07:23Z Article http://purl.org/eprint/type/JournalArticle 1754-1611 http://hdl.handle.net/1721.1/75371 Purcell, Oliver et al. “Temperature Dependence of ssrA-tag Mediated Protein Degradation.” Journal of Biological Engineering 6.1 (2012): 10. Web. https://orcid.org/0000-0002-2031-8871 en http://dx.doi.org/10.1186/1754-1611-6-10 Journal of Biological Engineering Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 Oliver Purcell et al.; licensee BioMed Central Ltd. application/pdf BioMed Central Ltd. BioMed Central Ltd |
| spellingShingle | Purcell, Oliver Grierson, Claire S Bernardo, Mario Savery, Nigel J Temperature dependence of ssrA-tag mediated protein degradation |
| title | Temperature dependence of ssrA-tag mediated protein degradation |
| title_full | Temperature dependence of ssrA-tag mediated protein degradation |
| title_fullStr | Temperature dependence of ssrA-tag mediated protein degradation |
| title_full_unstemmed | Temperature dependence of ssrA-tag mediated protein degradation |
| title_short | Temperature dependence of ssrA-tag mediated protein degradation |
| title_sort | temperature dependence of ssra tag mediated protein degradation |
| url | http://hdl.handle.net/1721.1/75371 https://orcid.org/0000-0002-2031-8871 |
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