Temperature dependence of ssrA-tag mediated protein degradation

Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be...

Full description

Bibliographic Details
Main Authors: Purcell, Oliver, Grierson, Claire S, Bernardo, Mario, Savery, Nigel J
Other Authors: Massachusetts Institute of Technology. Research Laboratory of Electronics
Format: Article
Language:English
Published: BioMed Central Ltd. 2012
Online Access:http://hdl.handle.net/1721.1/75371
https://orcid.org/0000-0002-2031-8871