In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging
Author Manuscript 2012 March 1.
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2012
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| Online Access: | http://hdl.handle.net/1721.1/75406 https://orcid.org/0000-0003-3055-797X https://orcid.org/0000-0002-7832-7156 |
| _version_ | 1811091370385866752 |
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| author | Artzi, Natalie Oliva, Nuria Puron, Cristina Shitreet, Sagi bon Ramos, Adriana Artzi, Shay Groothuis, Adam R. Sahagian, Gary Edelman, Elazer R |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Artzi, Natalie Oliva, Nuria Puron, Cristina Shitreet, Sagi bon Ramos, Adriana Artzi, Shay Groothuis, Adam R. Sahagian, Gary Edelman, Elazer R |
| author_sort | Artzi, Natalie |
| collection | MIT |
| description | Author Manuscript 2012 March 1. |
| first_indexed | 2024-09-23T15:01:20Z |
| format | Article |
| id | mit-1721.1/75406 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:01:20Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/754062022-01-21T02:05:35Z In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging Artzi, Natalie Oliva, Nuria Puron, Cristina Shitreet, Sagi bon Ramos, Adriana Artzi, Shay Groothuis, Adam R. Sahagian, Gary Edelman, Elazer R Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Artzi, Natalie Oliva, Nuria Puron, Cristina Shitreet, Sagi bon Ramos, Adriana Edelman, Elazer R. Author Manuscript 2012 March 1. The design of erodible biomaterials relies on the ability to program the in vivo retention time, which necessitates real-time monitoring of erosion. However, in vivo performance cannot always be predicted by traditional determination of in vitro erosion[superscript 1, 2] , and standard methods sacrifice samples or animals[superscript 3], preventing sequential measures of the same specimen. We harnessed non-invasive fluorescence imaging to sequentially follow in vivo material-mass loss to model the degradation of materials hydrolytically (PEG:dextran hydrogel) and enzymatically (collagen). Hydrogel erosion rates in vivo and in vitro correlated, enabling the prediction of in vivo erosion of new material formulations from in vitro data. Collagen in vivo erosion was used to infer physiologic in vitro conditions that mimic erosive in vivo environments. This approach enables rapid in vitro screening of materials, and can be extended to simultaneously determine drug release and material erosion from a drug-eluting scaffold, or cell viability and material fate in tissue-engineering formulations. National Institutes of Health (U.S.) (GM/HL 49039) National Institutes of Health (U.S.) (UL1 RR 025758) 2012-12-12T15:35:54Z 2012-12-12T15:35:54Z 2011-08 2011-03 Article http://purl.org/eprint/type/JournalArticle 1476-1122 1476-4660 http://hdl.handle.net/1721.1/75406 Artzi, Natalie et al. “In Vivo and in Vitro Tracking of Erosion in Biodegradable Materials Using Non-invasive Fluorescence Imaging.” Nature Materials 10.9 (2011): 704–709. https://orcid.org/0000-0003-3055-797X https://orcid.org/0000-0002-7832-7156 en_US http://dx.doi.org/10.1038/nmat3095 Nature Materials Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Artzi, Natalie Oliva, Nuria Puron, Cristina Shitreet, Sagi bon Ramos, Adriana Artzi, Shay Groothuis, Adam R. Sahagian, Gary Edelman, Elazer R In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging |
| title | In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging |
| title_full | In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging |
| title_fullStr | In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging |
| title_full_unstemmed | In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging |
| title_short | In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging |
| title_sort | in vivo and in vitro tracking of erosion in biodegradable materials using non invasive fluorescence imaging |
| url | http://hdl.handle.net/1721.1/75406 https://orcid.org/0000-0003-3055-797X https://orcid.org/0000-0002-7832-7156 |
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