Engineering humanized mice for improved hematopoietic reconstitution
Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discus...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2013
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| Online Access: | http://hdl.handle.net/1721.1/76183 https://orcid.org/0000-0002-5687-6154 |
| _version_ | 1826210529149976576 |
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| author | Drake, Adam Chen, Jianzhu Chen, Qingfeng |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Drake, Adam Chen, Jianzhu Chen, Qingfeng |
| author_sort | Drake, Adam |
| collection | MIT |
| description | Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications. |
| first_indexed | 2024-09-23T14:51:05Z |
| format | Article |
| id | mit-1721.1/76183 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:51:05Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/761832022-10-01T22:55:32Z Engineering humanized mice for improved hematopoietic reconstitution Drake, Adam Chen, Jianzhu Chen, Qingfeng Singapore-MIT Alliance in Research and Technology (SMART) Koch Institute for Integrative Cancer Research at MIT Chen, Jianzhu Drake, Adam Chen, Jianzhu Chen, Qingfeng Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications. Massachusetts Institute of Technology. Singapore-MIT Alliance in Research and Technology (SMART) Ragon Institute of MGH, MIT and Harvard 2013-01-08T14:47:19Z 2013-01-08T14:47:19Z 2012-05 Article http://purl.org/eprint/type/JournalArticle 1672-7681 2042-0226 http://hdl.handle.net/1721.1/76183 Drake, Adam C, Qingfeng Chen, and Jianzhu Chen. “Engineering Humanized Mice for Improved Hematopoietic Reconstitution.” Cellular and Molecular Immunology 9.3 (2012): 215–224. https://orcid.org/0000-0002-5687-6154 en_US http://dx.doi.org/10.1038/cmi.2012.6 Cellular and Molecular Immunology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group Chen via Courtney Crummett |
| spellingShingle | Drake, Adam Chen, Jianzhu Chen, Qingfeng Engineering humanized mice for improved hematopoietic reconstitution |
| title | Engineering humanized mice for improved hematopoietic reconstitution |
| title_full | Engineering humanized mice for improved hematopoietic reconstitution |
| title_fullStr | Engineering humanized mice for improved hematopoietic reconstitution |
| title_full_unstemmed | Engineering humanized mice for improved hematopoietic reconstitution |
| title_short | Engineering humanized mice for improved hematopoietic reconstitution |
| title_sort | engineering humanized mice for improved hematopoietic reconstitution |
| url | http://hdl.handle.net/1721.1/76183 https://orcid.org/0000-0002-5687-6154 |
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