Transport Model for Microfluidic Device for Cell Culture and Tissue Development
In recent years, microfluidic devices have emerged as a platform in which to culture tissue for various applications such as drug discovery, toxicity testing, and fundamental investigations of cell-cell interactions. We examine the transport phenomena associated with gradients of soluble factors and...
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| Format: | Article |
| Language: | en_US |
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Cambridge University Press (Materials Research Society)
2013
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| Online Access: | http://hdl.handle.net/1721.1/76193 https://orcid.org/0000-0003-0290-3054 https://orcid.org/0000-0002-1801-5548 |
| _version_ | 1826201414423019520 |
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| author | Inamdar, Niraj K. Griffith, Linda G. Borenstein, Jeffrey T. |
| author2 | Charles Stark Draper Laboratory |
| author_facet | Charles Stark Draper Laboratory Inamdar, Niraj K. Griffith, Linda G. Borenstein, Jeffrey T. |
| author_sort | Inamdar, Niraj K. |
| collection | MIT |
| description | In recent years, microfluidic devices have emerged as a platform in which to culture tissue for various applications such as drug discovery, toxicity testing, and fundamental investigations of cell-cell interactions. We examine the transport phenomena associated with gradients of soluble factors and oxygen in a microfluidic device for co-culture. This work focuses on emulating conditions known to be important in sustaining a viable culture of cells. Critical parameters include the flow and the resulting shear stresses, the transport of various soluble factors throughout the flow media, and the mechanical arrangement of the cells in the device. Using analytical models derived from first principles, we investigate interactions between flow conditions and transport in a microfluidic device. A particular device of interest is a bilayer configuration in which critical solutes such as oxygen are delivered through the media into one channel, transported across a nanoporous membrane, and consumed by cells cultured in another. The ability to control the flow conditions in this membrane bilayer device to achieve sufficient oxygenation without shear damage is shown to be superior to the case present in a single channel system. Using the results of these analyses, a set of criteria that characterize the geometric and transport properties of a robust microfluidic device are provided. |
| first_indexed | 2024-09-23T11:51:32Z |
| format | Article |
| id | mit-1721.1/76193 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:51:32Z |
| publishDate | 2013 |
| publisher | Cambridge University Press (Materials Research Society) |
| record_format | dspace |
| spelling | mit-1721.1/761932022-09-27T22:22:52Z Transport Model for Microfluidic Device for Cell Culture and Tissue Development Inamdar, Niraj K. Griffith, Linda G. Borenstein, Jeffrey T. Charles Stark Draper Laboratory Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Inamdar, Niraj K. Griffith, Linda G. Borenstein, Jeffrey T. In recent years, microfluidic devices have emerged as a platform in which to culture tissue for various applications such as drug discovery, toxicity testing, and fundamental investigations of cell-cell interactions. We examine the transport phenomena associated with gradients of soluble factors and oxygen in a microfluidic device for co-culture. This work focuses on emulating conditions known to be important in sustaining a viable culture of cells. Critical parameters include the flow and the resulting shear stresses, the transport of various soluble factors throughout the flow media, and the mechanical arrangement of the cells in the device. Using analytical models derived from first principles, we investigate interactions between flow conditions and transport in a microfluidic device. A particular device of interest is a bilayer configuration in which critical solutes such as oxygen are delivered through the media into one channel, transported across a nanoporous membrane, and consumed by cells cultured in another. The ability to control the flow conditions in this membrane bilayer device to achieve sufficient oxygenation without shear damage is shown to be superior to the case present in a single channel system. Using the results of these analyses, a set of criteria that characterize the geometric and transport properties of a robust microfluidic device are provided. National Institute of Biomedical Imaging and Bioengineering (U.S.) (grant # 5R01EB010246-02) 2013-01-08T16:12:20Z 2013-01-08T16:12:20Z 2011 2010-11 Article http://purl.org/eprint/type/ConferencePaper 1946-4274 http://hdl.handle.net/1721.1/76193 Inamdar, Niraj, Linda Griffith, and Jeffrey T. Borenstein. “Transport Model for Microfluidic Device for Cell Culture and Tissue Development.” MRS Proceedings 1299 (2011): 35-40. https://orcid.org/0000-0003-0290-3054 https://orcid.org/0000-0002-1801-5548 en_US http://dx.doi.org/10.1557/opl.2011.467 MRS Proceedings Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Cambridge University Press (Materials Research Society) MIT Web Domain |
| spellingShingle | Inamdar, Niraj K. Griffith, Linda G. Borenstein, Jeffrey T. Transport Model for Microfluidic Device for Cell Culture and Tissue Development |
| title | Transport Model for Microfluidic Device for Cell Culture and Tissue Development |
| title_full | Transport Model for Microfluidic Device for Cell Culture and Tissue Development |
| title_fullStr | Transport Model for Microfluidic Device for Cell Culture and Tissue Development |
| title_full_unstemmed | Transport Model for Microfluidic Device for Cell Culture and Tissue Development |
| title_short | Transport Model for Microfluidic Device for Cell Culture and Tissue Development |
| title_sort | transport model for microfluidic device for cell culture and tissue development |
| url | http://hdl.handle.net/1721.1/76193 https://orcid.org/0000-0003-0290-3054 https://orcid.org/0000-0002-1801-5548 |
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