Cell Elasticity Determines Macrophage Function
Macrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different orig...
| Main Authors: | , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/76586 |
| _version_ | 1826212963417063424 |
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| author | Patel, Naimish R. Bole, Medhavi Chen, Cheng Hardin, Charles C. Kho, Alvin T. Mih, Justin Deng, Linhong Butler, James Tschumperlin, Daniel Fredberg, Jeffrey J. Krishnan, Ramaswamy Koziel, Henry |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Patel, Naimish R. Bole, Medhavi Chen, Cheng Hardin, Charles C. Kho, Alvin T. Mih, Justin Deng, Linhong Butler, James Tschumperlin, Daniel Fredberg, Jeffrey J. Krishnan, Ramaswamy Koziel, Henry |
| author_sort | Patel, Naimish R. |
| collection | MIT |
| description | Macrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different origins and species, we report that macrophage elasticity is a major determinant of innate macrophage function. Macrophage elasticity is modulated not only by classical biologic activators such as LPS and IFN-γ, but to an equal extent by substrate rigidity and substrate stretch. Macrophage elasticity is dependent upon actin polymerization and small rhoGTPase activation, but functional effects of elasticity are not predicted by examination of gene expression profiles alone. Taken together, these data demonstrate an unanticipated role for cell elasticity as a common pathway by which mechanical and biologic factors determine macrophage function. |
| first_indexed | 2024-09-23T15:40:56Z |
| format | Article |
| id | mit-1721.1/76586 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:40:56Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/765862022-10-02T03:22:25Z Cell Elasticity Determines Macrophage Function Patel, Naimish R. Bole, Medhavi Chen, Cheng Hardin, Charles C. Kho, Alvin T. Mih, Justin Deng, Linhong Butler, James Tschumperlin, Daniel Fredberg, Jeffrey J. Krishnan, Ramaswamy Koziel, Henry Harvard University--MIT Division of Health Sciences and Technology Kho, Alvin T. Macrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different origins and species, we report that macrophage elasticity is a major determinant of innate macrophage function. Macrophage elasticity is modulated not only by classical biologic activators such as LPS and IFN-γ, but to an equal extent by substrate rigidity and substrate stretch. Macrophage elasticity is dependent upon actin polymerization and small rhoGTPase activation, but functional effects of elasticity are not predicted by examination of gene expression profiles alone. Taken together, these data demonstrate an unanticipated role for cell elasticity as a common pathway by which mechanical and biologic factors determine macrophage function. National Heart, Lung, and Blood Institute National Institute of Allergy and Infectious Diseases (U.S.) 2013-01-23T21:06:11Z 2013-01-23T21:06:11Z 2012-09 2012-02 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/76586 Patel, Naimish R. et al. “Cell Elasticity Determines Macrophage Function.” Ed. Laurel L. Lenz. PLoS ONE 7.9 (2012): e41024. Web. en_US http://dx.doi.org/10.1371/journal.pone.0041024 PLoS One Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Patel, Naimish R. Bole, Medhavi Chen, Cheng Hardin, Charles C. Kho, Alvin T. Mih, Justin Deng, Linhong Butler, James Tschumperlin, Daniel Fredberg, Jeffrey J. Krishnan, Ramaswamy Koziel, Henry Cell Elasticity Determines Macrophage Function |
| title | Cell Elasticity Determines Macrophage Function |
| title_full | Cell Elasticity Determines Macrophage Function |
| title_fullStr | Cell Elasticity Determines Macrophage Function |
| title_full_unstemmed | Cell Elasticity Determines Macrophage Function |
| title_short | Cell Elasticity Determines Macrophage Function |
| title_sort | cell elasticity determines macrophage function |
| url | http://hdl.handle.net/1721.1/76586 |
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