Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy
Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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IOP Publishing
2013
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| Online Access: | http://hdl.handle.net/1721.1/76615 |
| _version_ | 1826193263848062976 |
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| author | Sung, Yongjin Choi, Wonshik Lue, Niyom Dasari, Ramachandra Rao Yaqoob, Zahid |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Sung, Yongjin Choi, Wonshik Lue, Niyom Dasari, Ramachandra Rao Yaqoob, Zahid |
| author_sort | Sung, Yongjin |
| collection | MIT |
| description | Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells. |
| first_indexed | 2024-09-23T09:36:14Z |
| format | Article |
| id | mit-1721.1/76615 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:36:14Z |
| publishDate | 2013 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | mit-1721.1/766152022-09-26T12:35:44Z Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy Sung, Yongjin Choi, Wonshik Lue, Niyom Dasari, Ramachandra Rao Yaqoob, Zahid Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Spectroscopy Laboratory Sung, Yongjin Lue, Niyom Dasari, Ramachandra Rao Yaqoob, Zahid Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells. National Institute of Biomedical Imaging and Bioengineering (U.S.) (9P41EB015871-26A1) 2013-01-25T20:04:07Z 2013-01-25T20:04:07Z 2012-11 2012-06 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/76615 Sung Y, Choi W, Lue N, Dasari RR, Yaqoob Z (2012) Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy. PLoS ONE 7(11): e49502. en_US http://dx.doi.org/10.1371/journal.pone.0049502 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf IOP Publishing PLoS |
| spellingShingle | Sung, Yongjin Choi, Wonshik Lue, Niyom Dasari, Ramachandra Rao Yaqoob, Zahid Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy |
| title | Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy |
| title_full | Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy |
| title_fullStr | Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy |
| title_full_unstemmed | Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy |
| title_short | Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy |
| title_sort | stain free quantification of chromosomes in live cells using regularized tomographic phase microscopy |
| url | http://hdl.handle.net/1721.1/76615 |
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